Chromatin IP (ChIP) Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
CST provides multiple options for avoiding the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions that can damage both antibody epitopes and the integrity of the chromatin.
Chromatin immunoprecipitation, or ChIP, is a technique that uses antibodies to isolate specific DNA-binding proteins along with the bound DNA fragments from cells and tissues.
Multiplex Oligos for Illumina® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.a
Multiplex Oligos for Illumina® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.
Sonication ChIP Kit Performance Comparison
DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.
