| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 7004P4 | 50 ml |
|
|
| 7004P6 | 250 ml |
|
Product Information
(a) After incubation with horseradish peroxidase (HRP) conjugated secondary antibody, wash plates with PBS/0.05% Tween-20.
(b) Add 100 μl of TMB solution/well. Incubate for specific amount of time or until desired signal is achieved. See product datasheet for recommended incubation times for specific antibody pair.
(c) Add 100 μl of STOP solution.
(d) Read Absorbance at 450 nm.
TMB is a one component system that is ready to use from the bottle.
TMB (3,3', 5,5"-tetramethylbenzidine) is used as a substrate to generate detectable signal in ELISA. The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measureable color change that correlates with analyte level.
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