Intracellular Flow Cytometry Kit (Triton X-100) (#51995) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Storage

All components in this kit are stable for at least 12 months when stored at the recommended temperature and left unused. Formaldehyde fixative should be used within one month after opening. Upon receipt, #13616 and #39487 should be stored at 4°C. Remaining components should be stored at room temperature.

Product Description

The Intracellular Flow Cytometry Kit (Triton X-100) provides the supporting reagents needed to preserve protein states and enable antibodies to bind intracellular targets, for flow cytometric analysis of cells in suspension. Detergent-based permeabilization provides access to the nucleus and other intracellular organelles, without affecting fluorophores of direct conjugates added prior to fixation. This kit contains sufficient reagents for 100 individual samples when following the included protocol.

IMPORTANT: Please refer to the antibody product page to determine if it is validated for use in Flow Cytometry (F) and for information regarding appropriate antibody dilution. Some primary antibodies may require methanol permeabilization, which will be noted on the datasheet. Methanol is not included in this kit.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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