PMSF (#8553) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Information

Product Usage Information

For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use. Typically, a final concentration of 1 mM provides sufficient protease protection.

Storage

Store lyophilized at Room Temperature, protected from direct light. In lyophilized form, the chemical is stable for 24 months. Once in solution, PMSF can be stored at -20° C for up to 3 months, protected from light.

Product Description

Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation. Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer (#9803) and RIPA Buffer (#9806). Molecular Formula: C₇H₇FO₂S Molecular Weight: 174.19 CAS: 329-98-6

MW (kDa)	174.19
Molecular Formula	C₇H₇FO₂S

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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