Product Information
Store at room temperature. Protect from light. This product is stable for 12 months when stored at room temperature.
Safety Information: This product contains Dimethylformamide (DMF). DMF is rapidly absorbed through skin and lungs. It may cause harm to the unborn child. Avoid exposure during pregnancy. If exposed or concerned, get medical advice. See Safety Data Sheet (SDS).
Wear personal protective equipment. Avoid contact with skin. Do not handle until all safety precautions have been read and understood.
TrueBlack® Lipofuscin Autofluorescence Quencher reacts with lipofuscins to reduce autofluorescence. Consider using this reagent when working with tissue containing lipofuscin (brain, kidney, liver, heart, retina).
IMPORTANT:
Permeabilize sections as necessary using a buffer compatible with your primary reagent of choice. We recommend a gentle detergent such as Triton™ X-100 diluted to 0.3% in 1X Wash Buffer.
IMPORTANT: TrueBlack® Lipofuscin Autofluorescence Quencher is not compatible with detergent. Any steps involving detergent must be done before applying TrueBlack® Lipofuscin Autofluorescence Quencher.
Prepare TrueBlack® Lipofuscin Autofluorescence Quencher solution by diluting 1:20 in 70% ethanol. Vortex to mix.
NOTE: Quenching solution should be made fresh prior to use and discarded if precipitate is visible. We recommend heating the vial of stock solution of TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF to 70°C prior to dilution in order to avoid precipitate formation.
Immediately cover tissue sections with 100 µL - 200 µL of quenching solution for 30 seconds at room temperature.
IMPORTANT: Do not allow sections to dry out. Sections may tolerate longer incubations (up to 3 minutes) so long as they remain hydrated.
Protocol Id: 2567
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| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 92401S | 1 ml |
|
