Revision 2
Cell Signaling Technology

Orders: 877-616-CELL (2355) orders@cellsignal.com

Support: 877-678-TECH (8324)

Web: info@cellsignal.com cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

FC-L

:

:

:

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UniProt ID:

#P08758

Entrez-Gene Id:

308

Product Information

Storage

The components within the Annexin V-PE Early Apoptosis Detection Kit are stable for 1 year at 4°C. Protect from light.

Specificity / Sensitivity

Species Reactivity:

All Species Expected

Product Description

The Annexin V-PE Early Apoptosis Detection Kit enables researchers to identify early apoptotic cells within a cell population. Annexin V-PE conjugated protein binds to cell surfaces expressing phosphatidylserine, an early apotosis marker. Cells stained with 7-Aminoactinomycin D (7-AAD), a non-cell-permeable DNA dye, indicate necrotic cells. Cells stained with both 7-AAD and annexin V-PE demonstrate later stage apoptosis and early necrosis. This kit provides enough reagent to perform 100 assays, based on a 250 μl assay volume.

Background

Annexin V belongs to the annexin family of proteins consisting of over 160 members. All annexin family members share the same characteristic of Ca2+-dependent binding to negatively charged phospholipid surfaces (1). During early apoptosis, phosphatidylserine (PS) is translocated from the cytosolic side of the plasma membrane to the cellular surface. This translocation exposes PS to the extracellular environment with the plasma membrane left intact (2). Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).

  1. Boersma, H.H. et al. (2005) J Nucl Med 46, 2035-50.
  2. Vermes, I. et al. (1995) J Immunol Methods 184, 39-51.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is sold under license from Tau Technologies BV, U.S. Pat. 7,511,016 and foreign equivalents.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 2
#40295

Annexin V-PE Early Apoptosis Detection Kit

Flow Cytometry Image 1: Annexin V-PE Early Apoptosis Detection Kit Expand Image
Flow cytometric analysis of Jurkat cells, untreated (left) or treated with camptothecin (10 uM, 4 hr; right), using Annexin V-PE Early Apoptosis Detection Kit.