Annexin V-PE Early Apoptosis Detection Kit (#40295) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

FC-L

UniProt ID:

#P08758

Entrez-Gene Id:

308

Product Information

Storage

The components within the Annexin V-PE Early Apoptosis Detection Kit are stable for 1 year at 4°C. Protect from light.

Specificity / Sensitivity

Species Reactivity:

All Species Expected

Product Description

The Annexin V-PE Early Apoptosis Detection Kit enables researchers to identify early apoptotic cells within a cell population. Annexin V-PE conjugated protein binds to cell surfaces expressing phosphatidylserine, an early apotosis marker. Cells stained with 7-Aminoactinomycin D (7-AAD), a non-cell-permeable DNA dye, indicate necrotic cells. Cells stained with both 7-AAD and annexin V-PE demonstrate later stage apoptosis and early necrosis. This kit provides enough reagent to perform 100 assays, based on a 250 μl assay volume.

Background

Annexin V belongs to the annexin family of proteins consisting of over 160 members. All annexin family members share the same characteristic of Ca²⁺-dependent binding to negatively charged phospholipid surfaces (1). During early apoptosis, phosphatidylserine (PS) is translocated from the cytosolic side of the plasma membrane to the cellular surface. This translocation exposes PS to the extracellular environment with the plasma membrane left intact (2). Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

FC-L: Flow Cytometry (Live)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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This product is sold under license from Tau Technologies BV, U.S. Pat. 7,511,016 and foreign equivalents.

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