Product Information
Product Usage Information
Use as directed in the respective SimpleChIP® Chromatin IP kit and the CUT&RUN Assay kit protocols. Please note that if the following the SimpleChIP® Chromotin protocol there will be excess DNA Binding Buffer (#10007) to accommodate the CUT&RUN protocol.
Storage
Product Description
This product also is offered to conveniently provide additional ChIP buffer reagents for preparing, immunoprecipitating, washing, and eluting chromatin using our SimpleChIP® (#9002, #9003) and SimpleChIP® Plus (#9004, #9005) Enzymatic Chromatin IP Kits, as well as our SimpleChIP® Sonication Chromatin IP kit (#56383). These SimpleChIP® kits provide all the reagents required for performing the recommended of chromatin preparations (or optimizations) and chromatin immunoprecipitation (ChIP) assays, however there are instances where extra ChIP Buffer, ChIP Elution buffer, and NaCl are desired.
The DNA purification columns combine the convenience of spin-column technology with the selective binding properties of a uniquely designed silica membrane that allows for efficient recovery of DNA and removal of protein contaminants without the need for phenol/chloroform extractions and ethanol precipitations. After DNA purification, the enrichment of particular DNA sequences can be analyzed by a variety of methods.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
