PathScan® RP Androgen Receptor (Carboxy-terminal Antigen) Sandwich ELISA Kit (#57652) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Color	Storage Temp
Androgen Receptor Rabbit mAb (Carboxy-terminal Antigen) Coated Microwells	69500	96 tests		+4C
Androgen Receptor Rabbit Detection mAb	91058	1 ea	Red (Lyophilized)	+4C
HRP Diluent	13515	5.5 ml	Red	+4C
TMB Substrate	7004	11 ml		+4C
STOP Solution	7002	11 ml		+4C
Sealing Tape	54503	2 ea		+4C
ELISA Wash Buffer (20X)	9801	25 ml		+4C
Cell Lysis Buffer (10X)	9803	15 ml		-20C

*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Description

The rapid protocol (RP) PathScan^® RP Androgen Receptor (Carboxy-terminal Antigen) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of androgen receptor protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with androgen receptor protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of androgen receptor protein. Learn more about all of your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Specificity/Sensitivity

The PathScan^® RP Androgen Receptor (Carboxy-terminal Antigen) Sandwich ELISA Kit detects endogenous levels of androgen receptor protein. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. One ELISA antibody recognizes residues near the amino-terminal region of androgen receptor, while the other antibody recognizes the carboxy-terminal antigen that is present in full-length androgen receptor but not in the androgen receptor (V7 isoform) protein.

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).

Background References

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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PathScan is a registered trademark of Cell Signaling Technology, Inc.

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