WB, IP
H M R
Endogenous
254
Rabbit IgG
#O95477
19
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1262 of human ABCA1 protein.
Background
ATP-binding cassette (ABC) proteins are membrane-residing transporters that transport substrates across the membrane in an ATP-dependent manner. ABC substrates subject to active transport across the membrane include ions, amino acids, lipids, and sterols (1). ATP-Binding cassette sub-family A member 1 (ABCA1) is a member of the ABC family and functions to regulate phospholipid and cholesterol homeostasis. ABCA1, like most ABC transporters, contains two transmembrane domain bundles composed of six membrane-spanning helices and two nucleotide-binding domains. ABCA1 and its closest homolog, ABCA7, are 12A class members of ABCs, and both proteins function to transport cholesterol and phospholipids in an apolipoprotein A (apoA)-dependent manner (2,3). ABCA1 is expressed in a variety of tissues (4). Loss of function mutations in the human ABCA1 gene are linked to Tangier disease, a disorder characterized by high density lipoprotein (HDL) deficiency and cholesterol ester buildup in macrophages, as well as increased risk of atherosclerosis, consistent with ABCA1's function in maintaining cholesterol homeostasis and HDL formation (5-8). ABCA1 is also a candidate risk gene for late onset Alzheimer's disease (LOAD), a neurodegenerative disease that is also linked to altered cholesterol transport and metabolism (9). ABCA1 dysfunction may contribute directly to Alzheimer's disease (AD) pathogenesis by accelerating amyloid-β (Aβ) production, which is one of the pathological hallmarks of AD (9).
- Higgins, C.F. (1992) Annu Rev Cell Biol 8, 67-113.
- Abe-Dohmae, S. et al. (2004) J Biol Chem 279, 604-11.
- Wang, N. et al. (2003) J Biol Chem 278, 42906-12.
- Pereira, C.D. et al. (2018) J Alzheimers Dis 61, 463-85.
- Brooks-Wilson, A. et al. (1999) Nat Genet 22, 336-45.
- Bodzioch, M. et al. (1999) Nat Genet 22, 347-51.
- Rust, S. et al. (1999) Nat Genet 22, 352-5.
- Singaraja, R.R. et al. (2003) Arterioscler Thromb Vasc Biol 23, 1322-32.
- Lupton, M.K. et al. (2014) J Alzheimers Dis 38, 897-906.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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