WB, IP
H M R
Endogenous
32
Rabbit IgG
#Q9BV23
57406
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile121 of human ABHD6 protein.
Background
ABHD6 (α/β-Hydrolase domain-containing 6) is a monoacylglycerol lipase, ubiquitously expressed with the highest expression in brown adipose tissue, small intestine, and brain (1). A high-fat diet upregulates ABHD6 mRNA expression in small intestine and liver, and ABHD6 knockdown protects against high-fat diet-induced obesity, hepatic steatosis, and systemic insulin resistance (2). In addition, it has been shown that ABHD6 is a negative modulator of glucose-stimulated insulin secretion (3). In the central nervous system, ABHD6 is expressed postsynptically and degrades the endocannabinoid 2-arachidonoylglycerol (2-AG), an endogenous activator of cannabinoid receptors (4,5). Inhibitors of α/β-hydrolase domain 6 (ABHD6) have been actively pursued as a promising approach to treat inflammation, metabolic disorders, and epilepsy (2,6,7).
- Lord, C.C. et al. (2013) Biochim Biophys Acta 1831, 792-802.
- Thomas, G. et al. (2013) Cell Rep 5, 508-20.
- Zhao, S. et al. (2014) Cell Metab 19, 993-1007.
- Blankman, J.L. et al. (2007) Chem Biol 14, 1347-56.
- Marrs, W.R. et al. (2010) Nat Neurosci 13, 951-7.
- Alhouayek, M. et al. (2013) Proc Natl Acad Sci U S A 110, 17558-63.
- Naydenov, A.V. et al. (2014) Neuron 83, 361-71.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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