Revision 1

#3662Store at -20C

Cell Signaling Technology

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Support: 877-678-TECH (8324)

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IP, IHC-P, IF-IC, FC-FP

REACTIVITY:

H M R Mk B

SENSITIVITY:

Endogenous

MW (kDa):

280

SOURCE:

Rabbit

UniProt ID:

#Q13085, #O00763

Entrez-Gene Id:

31, 32

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:50
Immunofluorescence (Immunocytochemistry) 1:50
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Acetyl CoA Carboxylase Antibody detects endogenous levels of all isoforms of acetyl CoA carboxylase protein.

Species Reactivity:

Human, Mouse, Rat, Monkey, Bovine

Species predicted to react based on 100% sequence homology

Chicken, D. melanogaster, Pig

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl CoA carboxylase alpha1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 1
#3662

Acetyl-CoA Carboxylase Antibody

Western Blotting Image 1: Acetyl-CoA Carboxylase Antibody Expand Image
Western blot analysis of extracts from 293, HeLa, A431, NIH/3T3, L929, C6, H-4-IIE and BAEC cells, using Acetyl CoA Carboxylase Antibody.
Immunoprecipitation Image 1: Acetyl-CoA Carboxylase Antibody Expand Image
Immunprecipitation of Acetyl-CoA Carboxylase from AICAR treated C2C12 cell extracts using Acetyl CoA Carboxylase antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control.
Immunohistochemistry Image 1: Acetyl-CoA Carboxylase Antibody Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Acetyl CoA Carboxylase Antibody in the presence of control peptide (left) or AcetylCoA Carboxylase Blocking Peptide #1062 (right).
Immunofluorescence Image 1: Acetyl-CoA Carboxylase Antibody Expand Image
Confocal microscopic images of A431 cells showing cytoplasmic stain with Acetyl CoA Carboxylase Antibody (A) compared to an isotype control (B).
Flow Cytometry Image 1: Acetyl-CoA Carboxylase Antibody Expand Image
Flow cytometric analysis of untreated HeLa cells, using Acetyl CoA Carboxylase Antibody (blue) compared to a nonspecific negative control antibody (red).