Revision 1
Cell Signaling Technology

Orders: 877-616-CELL (2355) orders@cellsignal.com

Support: 877-678-TECH (8324)

Web: info@cellsignal.com cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IHC-P, IF-IC, FC-FP

REACTIVITY:

H Mk

SENSITIVITY:

Endogenous

MW (kDa):

110, 150

Source/Isotype:

Rabbit IgG

UniProt ID:

#P55265

Entrez-Gene Id:

103

Product Information

Product Usage Information

This product is the carrier free version of product #81284. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #81284

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total ADAR1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu419 of human ADAR1 protein.

Background

Post-transcriptional processing of RNAs, such as RNA editing, is an important mechanism by which diversity in RNA and protein is achieved that is not otherwise encoded by the genome (1,2). The most common form of RNA editing is the conversion of adenosine (A) into inosine (I) on double-stranded RNA by the adenosine deaminase acting on RNA (ADAR) family of proteins (1-3). Since inosine base pairs with cytidine, it is interpreted as a guanosine by the splicing and translational machinery, leading to alteration in the protein sequence, as well as generation of splicing isoforms (1,4-6). A-to-I editing can also influence RNA sequence recognition by RNA-binding proteins and non-coding RNA, such as miRNAs, affecting subsequent RNA processing, stability, and protein expression levels (2).

ADAR1 is ubiquitously expressed with two known isoforms, ADAR1L (p150) and ADAR1S (p110), resulting from transcription using alternative promoters and start codons. ADAR1S is constitutively expressed in the nucleus, while ADAR1L is interferon-inducible and present in both the nucleus and the cytoplasm. The induction of ADAR1L in response to cellular stress and viral infection suggests a role for RNA editing in the innate immune response (1,7). In addition, ADAR1 is essential in mammalian development, particularly in hematopoiesis and suppression of interferon signaling to protect hematopoietic stem cells from destruction in fetal liver and adult bone marrow (8,9).

  1. Zinshteyn, B. and Nishikura, K. (2009) Wiley Interdiscip Rev Syst Biol Med 1, 202-9.
  2. Nishikura, K. (2006) Nat Rev Mol Cell Biol 7, 919-31.
  3. Bass, B.L. (2002) Annu Rev Biochem 71, 817-46.
  4. Reenan, R.A. (2001) Trends Genet 17, 53-6.
  5. Maas, S. et al. (2006) RNA Biol 3, 1-9.
  6. Rueter, S.M. et al. (1999) Nature 399, 75-80.
  7. Patterson, J.B. and Samuel, C.E. (1995) Mol Cell Biol 15, 5376-88.
  8. Iizasa, H. and Nishikura, K. (2009) Nat Immunol 10, 16-8.
  9. Hartner, J.C. et al. (2009) Nat Immunol 10, 109-15.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 1
#95404

ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free)

Western Blotting Image 1: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Western blot analysis of extracts from various cell lines using ADAR1 (E6X9R) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, the HCT 116 ADAR1 knockout (KO) cells do not show expression of ADAR1. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human appendix using ADAR1 (E6X9R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ADAR1 (E6X9R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using ADAR1 (E6X9R) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using ADAR1 (E6X9R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded HCT 116 cell pellet, wild-type (left, positive) or ADAR1 knockout (right, low-expressing), using ADAR1 (E6X9R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Immunohistochemical analysis of paraffin-embedded normal human prostate using ADAR1 (E6X9R) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Confocal immunofluorescent analysis of HCT 116 cells, either wild-type (left, positive) or ADAR1 knockout (right, negative), using ADAR1 (E6X9R) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 650 Phalloidin #12956 (red). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: ADAR1 (E6X9R) XP® Rabbit mAb (BSA and Azide Free) Expand Image
Flow cytometric analysis of HCT 116 cells, wild-type (green) or ADAR1 knockout (blue), using ADAR1 (E6X9R) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.