WB, IP, ChIP, ChIP-seq
H
Endogenous
100
Rabbit IgG
#O14867
571
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human BACH1 protein.
Background
BTB and CNC homology 1 (BACH1) is a basic leucine zipper transcription factor that is widely expressed in mammalian tissues (1). Through its roles in both transcriptional activation and repression, BACH1 regulates a wide range of cellular processes, including senescence, heme homeostasis, oxidative stress response, and the cell cycle (2-5). In competition with NRF2, BACH1 regulates ferroptosis; BACH1 promotes the process while NRF2 inhibits it (6,7). BACH1 heterodimerizes with Maf proteins (8). These heterodimers bind to Maf recognition elements at the promoters of oxidative stress response genes, such as heme oxygenase-1 (HMOX1) and NAD(P)H:quinone oxidoreductase 1 (NQO1) (9,10). As a transcriptional repressor, BACH1 recruits nuclear receptor corepressor 1 (NCoR1) as well as histone deacetylase 1 (HDAC1) (3). BACH1 has recently been implicated as a driver of cancer metastasis and is a potential therapeutic target in breast and lung cancer (11-13).
- Zhang, X. et al. (2018) Oxid Med Cell Longev 2018, 1347969.
- Oyake, T. et al. (1996) Mol Cell Biol 16, 6083-95.
- Dohi, Y. et al. (2008) Nat Struct Mol Biol 15, 1246-54.
- Ogawa, K. et al. (2001) EMBO J 20, 2835-43.
- Warnatz, H.J. et al. (2011) J Biol Chem 286, 23521-32.
- Nishizawa, H. et al. (2023) FEBS J 290, 1688-1704.
- Nishizawa, H. et al. (2020) J Biol Chem 295, 69-82.
- Igarashi, K. et al. (2021) J Biol Chem 297, 101032.
- Sun, J. et al. (2002) EMBO J 21, 5216-24.
- Dhakshinamoorthy, S. et al. (2005) J Biol Chem 280, 16891-900.
- Lee, J. et al. (2019) Nature 568, 254-258.
- Wiel, C. et al. (2019) Cell 178, 330-345.e22.
- Padilla, J. and Lee, J. (2021) Cells 10, 634.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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