WB, IP, ChIP, ChIP-seq, C&R, C&T
H M R Mk
Endogenous
70
Rabbit IgG
#Q14781
84733
Product Information
Product Usage Information
For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
| Chromatin IP-seq | 1:100 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human CBX2 protein.
Background
The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyl-transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). Chromobox protein homolog 2 (CBX2) is a core component of the PRC1 complex, which binds to the H3K27me3 mark and recruits PRC1 for transcriptional repression (7). CBX2 is frequently overexpressed in various cancers, including breast, ovarian, and AR-independent prostate cancer, where it is associated with poor prognosis (8-14). Furthermore, elevated CBX2 expression levels correlate with larger tumor size, increased lymph node metastasis, and positive human epidermal growth factor receptor 2 (HER-2) status in breast cancer (8). Mutations in CBX2 have also been attributed to 46,XY disorders of sex development (DSD), at least partially due to misregulation of the NR5A1/SF1 gene (15,16).
- Boyer, L.A. et al. (2006) Nature 441, 349-53.
- Lee, T.I. et al. (2006) Cell 125, 301-13.
- Park, I.K. et al. (2003) Nature 423, 302-5.
- Molofsky, A.V. et al. (2003) Nature 425, 962-7.
- Tavares, L. et al. (2012) Cell 148, 664-78.
- Gao, Z. et al. (2012) Mol Cell 45, 344-56.
- Kaustov, L. et al. (2011) J Biol Chem 286, 521-9.
- Chen, W.Y. et al. (2017) Oncol Lett 13, 1149-1156.
- Liang, Y.K. et al. (2017) Oncotarget 8, 92375-92387.
- Piqué, D.G. et al. (2019) Br J Cancer 120, 746-753.
- Zheng, S. et al. (2019) Am J Transl Res 11, 1668-1682.
- Iqbal, M.A. et al. (2021) Mol Oncol 15, 1450-1465.
- Wheeler, L.J. et al. (2018) Oncogenesis 7, 92.
- Clermont, P.L. et al. (2016) Clin Epigenetics 8, 16.
- Biason-Lauber, A. et al. (2009) Am J Hum Genet 84, 658-63.
- Sproll, P. et al. (2019) Sci Rep 9, 16552.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq C&R: CUT&RUN C&T: CUT&Tag
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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