WB, IHC-Bond, IHC-P, IF-IC
H Mk
Endogenous
60-85
Rabbit IgG
#P42081
942
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #76755.
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro239 of human CD86 protein.
Background
CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
- Freeman, G.J. et al. (1989) J Immunol 143, 2714-22.
- Azuma, M. et al. (1993) Nature 366, 76-9.
- Adams, A.B. et al. (2016) J Immunol 197, 2045-50.
- Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5.
- Gimmi, C.D. et al. (1991) Proc Natl Acad Sci U S A 88, 6575-9.
- Harding, F.A. et al. (1992) Nature 356, 607-9.
- Collins, A.V. et al. (2002) Immunity 17, 201-10.
- Linsley, P.S. et al. (1991) J Exp Med 174, 561-9.
- Krummel, M.F. and Allison, J.P. (1995) J Exp Med 182, 459-65.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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