WB, IP, IHC-P, IF-IC
H
Endogenous
38
Rabbit IgG
#Q99626
1045
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:1000 - 1:4000 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #23083.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CDX2 protein.
Background
CDX2, a homeobox domain-containing transcription factor, is a master regulator of the trophectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development (1). CDX2 is also involved in intestinal development (2), and gain of expression or loss of expression has been associated with various human malignancies, such as Barrett's esophagus (3) and colorectal cancer (4,5). Mouse embryonic stem cells deficient in CDX2 display limited hematopoietic progenitor development and altered Hox gene expression (6), pointing to a role for CDX2 in Hox gene regulation. CDX2 is also implicated in the aberrant expression of Hox genes in human AML cell lines (7).
- Ralston, A. and Rossant, J. (2008) Dev Biol 313, 614-29.
- Traber, P.G. and Silberg, D.G. (1996) Annu Rev Physiol 58, 275-97.
- Shi, X.Y. et al. (2008) Am J Clin Pathol 129, 571-7.
- Bakaris, S. et al. (2008) Histol Histopathol 23, 1043-7.
- Guo, R.J. et al. (2004) Cancer Biol Ther 3, 593-601.
- Wang, Y. et al. (2008) Proc Natl Acad Sci U S A 105, 7756-61.
- Fröhling, S. et al. (2007) Cell Cycle 6, 2241-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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