Revision 1

#17798Store at -20C

Cell Signaling Technology

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Support: 877-678-TECH (8324)

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB, IF-IC

REACTIVITY:

H Mk

SENSITIVITY:

Endogenous

MW (kDa):

68

SOURCE:

Rabbit

UniProt ID:

#Q13283

Entrez-Gene Id:

10146

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

G3BP1 Antibody recognizes endogenous levels of total G3BP1 protein. This antibody does not cross-react with G3BP2 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val218 of human G3BP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ras GTPase-activating protein-binding protein 1, also known as GAP SH3 domain-binding protein 1 (G3BP1), was identified as a protein that interacts with the SH3 domain of Ras GTPase-activating protein (RasGap) (1). G3BP1 is involved in the regulation of multiple cellular processes, including mRNA decay and inhibition of translation initiation (2). Furthermore, G3BP1 is essential for the assembly of stress granules (SGs) and functions as an SG-nucleating protein (3). Research studies show that arginine demethylation of G3BP1 promotes SG assembly during oxidative stress (4).

  1. Parker, F. et al. (1996) Mol Cell Biol 16, 2561-9.
  2. Fischer-Kešo, R. et al. (2014) Mol Cell Biol 34, 4244-56.
  3. Kedersha, N. et al. (2016) J Cell Biol 212, 845-60.
  4. Tsai, W.C. et al. (2016) J Biol Chem 291, 22671-85.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 1
#17798

G3BP1 Antibody

Western Blotting Image 1: G3BP1 Antibody Expand Image
Western blot analysis of extracts from 293T cells, either wild type (+/+) or G3BP1 knockout (-/-), using G3BP1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: G3BP1 Antibody Expand Image
Western blot analysis of extracts from various cell lines using G3BP1 Antibody.
Immunofluorescence Image 1: G3BP1 Antibody Expand Image
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or serum-starved and then treated with sodium arsenite (500 μM, 30 min; right), using G3BP1 Antibody (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).