WB, IHC-Bond, IHC-P
H
Endogenous
70-80
Rabbit IgG
#P15509
1438
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
IHC Leica Bond | 1:200 - 1:800 |
Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #63107.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human GM-CSF Receptor α protein.
Background
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells (1). GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor (2). Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival (3,4). Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction (5). Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling (1,6,7). Multiple isoforms of GM-CSF Receptor α have been described.
- Barreda, D.R. et al. (2004) Dev Comp Immunol 28, 509-54.
- Hansen, G. et al. (2008) Cell 134, 496-507.
- Doyle, S.E. and Gasson, J.C. (1998) Blood 92, 867-76.
- Lopez, A.F. et al. (2010) IUBMB Life 62, 509-18.
- McClure, B.J. et al. (2003) Blood 101, 1308-15.
- Raines, M.A. et al. (1991) Proc Natl Acad Sci U S A 88, 8203-7.
- Geijsen, N. et al. (2001) Cytokine Growth Factor Rev 12, 19-25.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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