WB, IP, IF-IC, FC-FP
H M R Mk
Endogenous
34, 40
Rabbit IgG
#P09651
3178
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly209 of human hnRNP A1 protein.
Background
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10).
- Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
-
Rooke, N. et al. (2003)
cite>Mol Cell Biol /cite> 23, 1874-84. -
Guil, S. et al. (2003)
cite>Mol Cell Biol /cite> 23, 2927-41. - Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53.
- Patry, C. et al. (2003) Cancer Res 63, 7679-88.
-
Piñol-Roma, S. and Dreyfuss, G. (1992)
cite>Nature /cite> 355, 730-2. -
Siomi, M.C. et al. (1997)
cite>J Cell Biol /cite> 138, 1181-92. - Black, A.C. et al. (1996) Virus Genes 12, 275-85.
- Hadian, K. et al. (2009) J Biol Chem 284, 33384-91.
-
Monette, A. et al. (2009)
cite>J Biol Chem /cite> 284, 31350-62.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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