| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 9092T | 1 Kit (6 x 20 microliters) |
|
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| Oct-4A (C30A3) Rabbit mAb 2840 | 20 µl |
|
H M | 45 | Rabbit IgG |
| Sox2 (D6D9) XP® Rabbit mAb 3579 | 20 µl |
|
H | 35 | Rabbit |
| Nanog (D73G4) XP® Rabbit mAb 4903 | 20 µl |
|
H | 42 | Rabbit IgG |
| LIN28A (D84C11) XP® Rabbit mAb 3695 | 20 µl |
|
H | 26 | Rabbit IgG |
| KLF4 Antibody 4038 | 20 µl |
|
H M Mk | 65 | Rabbit |
| c-Myc (D84C12) Rabbit mAb 5605 | 20 µl |
|
H M R | 57-65 | Rabbit IgG |
Product Information
Monoclonal antibodies are produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A, a synthetic peptide corresponding to residues surrounding Gly179 of human Sox2, a synthetic peptide corresponding to residues near the amino terminus of human nanog protein, a synthetic peptide corresponding to residues near the carboxy terminus of human LIN28A, and a synthetic peptide corresponding
to residues near the amino terminus of c-Myc. Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to residues near the amino terminus of human KLF4. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.
Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells.
Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated.
SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neuraminadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9).
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