WB, IHC-Bond, IHC-P
H
Endogenous
100
Rabbit IgG
#Q9Y4K0
4017
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:800 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LOXL2 protein.
Background
Lysyl oxidase-like 2 (LOXL2) is a secreted copper-dependent amine oxidase and a member of the lysyl oxidase family (1). It primarily catalyzes the oxidation of lysine (or hydroxylysine) residues in collagen and elastin to form peptidyl aldehyde derivatives (1). These modifications are required for further cross-linking of target proteins to enhance extracellular matrix (ECM) stiffness. LOXL2 has been associated with progression and metastases of many cancer types, including breast cancer and fibrotic disease, all through active remodeling of the ECM (2-5). Additionally, within the cell, LOXL2 has been shown to play a role in EMT, cell polarity, and transcriptional repression (6-8). LOXL2 has become an attractive target for therapeutic intervention in cancer treating, blocking metastasis, and halting fibrosis (9-10).
- Xiao, Q. and Ge, G. (2012) Cancer Microenviron 5, 261-73.
- Wang, C. et al. (2019) Neoplasia 21, 413-27.
- Lewis, M.A. et al. (1985) Med Pediatr Oncol 13, 97-100.
- Du, X.G. and Zhu, M.J. (2018) Onco Targets Ther 11, 2699-2708.
- Tian, J. et al. (2019) Onco Targets Ther 12, 8947-54.
- Cebrià-Costa, J.P. et al. (2020) Oncogene 39, 79-121.
- Wen, X. et al. (2018) Int J Mol Med 42, 3530-41.
- Moreno-Bueno, G. et al. (2011) EMBO Mol Med 3, 528-44.
- Puente, A. et al. (2019) Int J Mol Sci 20, pii: E1634. doi: 10.3390/ijms20071634.
- Trackman, P.C. (2016) Expert Opin Ther Targets 20, 935-45.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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