WB, IP, eCLIP
H Mk
Endogenous
30
Rabbit IgG
#O75569
8575
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
eCLIP | 1:200 |
For more information about eCLIP and the RBP-eCLIP kit, please visit Eclipse Bioinnovations.
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PACT protein.
Background
PACT (protein activator of protein kinase R) is a double stranded RNA binding protein and a cellular activator of PKR (protein kinase R), a kinase that mediates the antiviral and antiproliferative actions of interferon (1). Stress signals such as serum starvation and arsenite treatment induces PACT to heterodimerize with PKR, resulting in the phosphorylation of eIF-2α and inhibition of protein synthesis (1,2). PACT has also been shown to play a role in RNA-mediated gene silencing by stimulating the activity of Dicer, thereby affecting the efficiency of miRNA accumulation and siRNA gene silencing (3,4). More recently, PACT has been shown to interact with RIG-I, a sensor of viral nucleic acids, and stimulates RIG-I-induced type I interferon production (5). Researchers have found that mutations in PACT are associated with dystonia, a movement disorder where patients develop involuntary muscle contractions and postures (6-8).
- Patel, R.C. and Sen, G.C. (1998) EMBO J 17, 4379-90.
- Patel, C.V. et al. (2000) J Biol Chem 275, 37993-8.
- Lee, Y. et al. (2006) EMBO J 25, 522-32.
- Koscianska, E. et al. (2011) PLoS One 6, e28548.
- Kok, K.H. et al. (2011) Cell Host Microbe 9, 299-309.
- Bragg, D.C. et al. (2011) Neurobiol Dis 42, 136-47.
- Camargos, S. et al. (2008) Lancet Neurol 7, 207-15.
- Seibler, P. et al. (2008) Lancet Neurol 7, 380-1.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation eCLIP: eCLIP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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