WB
H M
Endogenous
75, 70
Rabbit IgG
#Q6L8Q7
201626
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PDE12 protein.
Background
2',5'-phosphodiesterase 12 (PDE12) is a ubiquitously expressed enzyme localized to the mitochondrial matrix. The catalytic activity of PDE12 is, in part, responsible for degradation of 2',5'-oligoadenylate second messengers (1-4). Through its ability to degrade 2',5'-oligoadenylates, PDE12 is considered to be a negative regulator of the interferon-induced OAS/RNase L antiviral signaling pathway. Indeed, either inhibition of PDE12 activity or loss of PDE12 expression has been shown to protect cells from viral pathogens by upregulating the activity of the OAS/RNase L pathway (1).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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