WB, IP
H M R Mk
Endogenous
78
Rabbit IgG
#Q05655
5580
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Xenopus, Bovine, Dog, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg216 of human PKCδ protein.
Background
Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7).
PKCδ is classified among the calcium-independent, diacylglycerol-activated "novel" members of the PKC superfamily, which includes PKCδ, ε, η, and θ. Unlike other PKC family members, whose activation appears to contribute to tumorigenesis, PKCδ appears to function as a tumor suppresor as down-regulation of this enzyme is associated with tumor progression (8). Like other conventional and novel PKCs, PKCδ is potently activated by diacylglycerol and phorbol ester and its kinase activity is modulated by phosphorylation within the conserved activation loop (Thr505) as well as the autophosphorylation site (Ser645) and hydrophobic, carboxy-terminal residue (Ser664) (9-11). Interestingly, PKCδ funtionality is uniquely regulated by phosphorylation at tyrosine residues by receptor tyrosine kinases, members of the Src kinase family, and c-Abl (9,12-14). For more information regarding PKCδ phosphorylation sites, please see PhosphoSitePlus® (www.phosphosite.org).
- Nishizuka, Y. (1984) Nature 308, 693-8.
- Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403.
- Mellor, H. and Parker, P.J. (1998) Biochem J 332 ( Pt 2), 281-92.
- Ron, D. and Kazanietz, M.G. (1999) FASEB J 13, 1658-76.
- Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep 1, 399-403.
- Baron, C.L. and Malhotra, V. (2002) Science 295, 325-8.
- Flynn, P. et al. (2000) J Biol Chem 275, 11064-70.
- Lu, Z. et al. (1997) Mol Cell Biol 17, 3418-28.
- Benes, C. and Soltoff, S.P. (2001) Am J Physiol Cell Physiol 280, C1498-510.
- Li, W. et al. (1997) J Biol Chem 272, 24550-5.
- Le Good, J.A. et al. (1998) Science 281, 2042-5.
- Morita, M. et al. (2008) J Biochem 143, 31-8.
- Denning, M.F. et al. (1996) J Biol Chem 271, 5325-31.
- Kronfeld, I. et al. (2000) J Biol Chem 275, 35491-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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