WB, IP, IF-IC
H M R
Endogenous
24
Rabbit
#P20340
5870
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Rab6 protein.
Background
Rab6 is a member of the Ras superfamily of small Rab GTPases implicated in endocytosis (1). The three distinct members of the Rab6 subfamily (Rab6A, Rab6A', and Rab6B) are structurally similar but likely exhibit non-overlapping functions (2,3). Rab6 localized to the Golgi (4) regulates retrograde transport of membrane-bound target proteins from the Golgi apparatus to the endoplasmic reticulum (5-7) or from the Golgi to the endosome during exocytotic transport (8). Rab6 interacts with microtubule motor proteins such as rabkinesin-6 (KIF20A) and dynein/dynactin complexes; Rab6-mediated transport requires a functionally intact microtubule system (9,10). Rab6 also regulates cytokinesis and cell cycle check point through interactions with Rab6 effector proteins, including the dynein/dynactin protein DCTN1 and the GTPase activating protein RABGAP1 (11,12).
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- Echard, A. et al. (2000) Mol Biol Cell 11, 3819-33.
- Del Nery, E. et al. (2006) Traffic 7, 394-407.
- Goud, B. et al. (1994) Ann N Y Acad Sci 733, 340-3.
- White, J. et al. (1999) J Cell Biol 147, 743-60.
- Opdam, F.J. et al. (2000) J Cell Sci 113 ( Pt 15), 2725-35.
- Martinez, O. et al. (1997) Proc Natl Acad Sci USA 94, 1828-33.
- Grigoriev, I. et al. (2007) Dev Cell 13, 305-14.
- Echard, A. et al. (1998) Science 279, 580-5.
- Young, J. et al. (2005) Mol Biol Cell 16, 162-77.
- Hill, E. et al. (2000) EMBO J 19, 5711-9.
- Miserey-Lenkei, S. et al. (2006) EMBO J 25, 278-89.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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