WB, IP, IF-IC
H M R Mk
Endogenous
23
Rabbit IgG
#P51151
9367
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human Rab9A protein.
Background
Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosomes and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
- Méresse, S. et al. (1995) J Cell Sci 108 (Pt 11), 3349-58.
- Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
- Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
- Méresse, S. et al. (1999) EMBO J 18, 4394-403.
- Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
- Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
- Lombardi, D. et al. (1993) EMBO J 12, 677-82.
- Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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