WB, IP, IF-IC
H M R
Endogenous
32
Rabbit IgG
#O43752
10228
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr140 of mouse syntaxin 6 protein.
Background
Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (1,2). The protein has an amino-terminal H1 domain followed by an H2 SNARE domain and a carboxy-terminal membrane anchor (3). Syntaxin 6 protein is localized to the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (3-5). Depending on cell type and SNARE parter, syntaxin 6 is involved in the regulation of GLUT4 trafficking, neutrophil exocytosis and granule secretion (6-10).
- Bock, J.B. et al. (2001) Nature 409, 839-841.
- Bock, J.B. et al. (1996) J. Biol. Chem. 271, 17961-17965.
- Wendler, F. and Tooze, S. (2001) Traffic 2, 606-611.
- Bock, J.B. et al. (1997) Mol. Biol. Cell 8, 1261-1271.
- Mallard, F. et al. (2002) J. Cell Biol. 156, 653-664.
- Perera, H.K. et al. (2003) Mol. Biol. Cell 14, 2946-2958.
- Shewan, A.M. et al. (2003) Mol. Biol. Cell 14, 973-986.
- Martín-Martín, B. et al. (2000) Blood 96, 2574-25783.
- Wendler, F. et al. (2001) Mol. Biol. Cell 12, 1699-1709.
- Kuliawat, R. et al. (2004) Mol. Biol. Cell 15, 1690-1701.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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