Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
---|---|---|---|---|
CD2AP Antibody | 2135 | 20 µl | 80 kDa | Rabbit  |
Claudin-1 (D5H1D) XP® Rabbit mAb | 13255 | 20 µl | 20 kDa | Rabbit IgG |
ZO-1 (D7D12) Rabbit mAb | 8193 | 20 µl | 220 kDa | Rabbit IgG |
ZO-2 Antibody | 2847 | 20 µl | 150 kDa | Rabbit  |
ZO-3 (D57G7) XP® Rabbit mAb | 3704 | 20 µl | 140 kDa | Rabbit IgG |
Afadin (D1Y3Z) Rabbit mAb | 13531 | 20 µl | 205 kDa | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat  |
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Description
The Tight Junction Antibody Sampler Kit provides an economical means to evaluate the presence of a number of proteins involved in tight junctions. The kit contains enough primary antibodies to perform two western blot experiments per primary antibody.
Storage
Background
Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and basolateral cell surfaces (reviewed in 1). Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier (2,3). Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 4). ZO-1 and ZO-2 are required for tight junction formation and function (5,6). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation (7-9). Exogenous expression of the amino terminal portion of ZO-3 exerts a dominant negative effect that interferes with assembly of tight junctions and adherens junctions (10). ZO-1 has been shown to interact with afadin prior to the formation of tight junctions (11). Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (12,13). CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (14-16). It plays a role in the formation of tight junctions in specialized cell types such as the slit diaphragm of the kidney glomerulus (17). CD2AP is also involved in the immunological synapse between CD2-expressing T cells and antigen presenting cells (18). Research studies have shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (16).
- Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35.
- Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28.
- Hewitt, K.J. et al. (2006) BMC Cancer 6, 186.
- Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11.
- Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47.
- Umeda, K. et al. (2006) Cell 126, 741-54.
- Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66.
- Traweger, A. et al. (2003) J Biol Chem 278, 2692-700.
- Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36.
- Wittchen, E.S. et al. (2000) J Cell Biol 151, 825-36.
- Ooshio, T. et al. (2010) J Biol Chem 285, 5003-12.
- Miyata, M. et al. (2009) J Cell Sci 122, 4319-29.
- Miyata, M. et al. (2009) J Biol Chem 284, 24595-609.
- Kirsch, K.H. et al. (1999) Proc Natl Acad Sci U S A 96, 6211-6.
- Kirsch, K.H. et al. (2001) J Biol Chem 276, 4957-63.
- Lynch, D.K. et al. (2003) J Biol Chem 278, 21805-13.
- Kawachi, H. et al. (2006) Nephrology (Carlton) 11, 274-81.
- Hutchings, N.J. et al. (2003) J Biol Chem 278, 22396-403.
Background References
Trademarks and Patents
Limited Uses
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