IHC-P
Product Information
Product Usage Information
1. To prepare the Vivid Purple working solution, to 5 ml SignalStain® Vivid Purple Diluent add 3 drops SignalStain® Vivid Purple Reagent 1, 3 drops SignalStain® Vivid Purple Reagent 2, 3 drops SignalStain® Vivid Purple Reagent 3, and 3 drops SignalStain® Vivid Purple Reagent 4. Mix well before use.
2. After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature for 2-15 minutes. Optimal development time should be determined by the investigator.
3. Wash slides for 5 minutes in water.
4. Counterstain, if desired.
5. Dehydrate, clear and permanently mount in a non-aqueous mounting medium. Do not use recycled alcohols with this product.
Prolonged incubation in alcohol may decrease sensitivity.
Unused working solution is stable up to 14 days if stored at 2-8ºC. Working solution may change color during this time with no impact on quality or intensity of the staining. If a precipitate forms, filter through a 0.45 μm filter.
Appropriate personal protective equipment should be worn when handling Vivid Purple. Dispose of in accordance with local regulations.
Storage
Product Description
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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