WB, IP
H
Endogenous
46
Rabbit
#Q9NUD5
85364
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln17 of human ZCCHC3 protein. Antibodies are purified by peptide affinity chromatography.
Background
ZCCHC3 belongs to the family of CCHC-type zinc-finger proteins (1). It binds to cGAS and acts as a cGAS co-sensor in recognizing double-stranded DNA (dsDNA), therefore, playing an essential role in innate immune response to DNA virus (2). ZCCHC3 was also shown to bind dsRNA and promote the binding of dsRNA by the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) RIG-I and MDA5, playing a critical role in RLR-mediated innate immune response to RNA virus (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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