Anti-mouse IgG, AP-linked Antibody (#7056) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

WB, ELISA

REACTIVITY:

SENSITIVITY:

MW (kDa):

Source/Isotype:

Goat

Product Information

Product Usage Information

Recommended Antibody Dilutions:

Western blotting 1:1000-1:3000

Storage

Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at -20°C. Do not aliquot the antibody. This product is stable for 60 months when stored at -20C.

Specificity / Sensitivity

Species Reactivity:

Mouse

Product Description

Affinity purified goat anti-mouse IgG (H&L) antibody is conjugated to calf intestinal alkaline phosphatase. This product has been optimized for use as a secondary antibody in Western blotting and ELISA applications.

Background

The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
This product detects both heavy and light chain (AP-anti-mouse-IgG (H+L), affinity purified.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

WB: Western Blotting ELISA: ELISA

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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