SignalStain® Boost IHC Detection Reagent (AP, Rat) (#15764) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

IHC-P

REACTIVITY:

SENSITIVITY:

MW (kDa):

Source/Isotype:

Goat

Product Information

Product Usage Information

1. Equilibrate SignalStain^® Boost IHC Detection Reagent (AP, Rat) to room temperature prior to applying to the slides.
2. Cover sections with 1-3 drops (40-120 µl), as needed, and incubate in a humidified chamber for 30 minutes at room temperature.
3. Wash slides and develop with any alkaline phosphatase-compatible substrate.

Storage

SignalStain^® Boost IHC Detection Reagent (AP, Rat) should be stored at 4°C and is stable for 12 months.

Specificity / Sensitivity

SignalStain^® Boost IHC Detection Reagent (AP, Rat) can be used to detect rat primary antibodies in immunohistochemical assays.

Species Reactivity:

Rat

Product Description

SignalStain^® Boost IHC Detection Reagent (AP, Rat) is a ready-to-use solution intended for use in immunohistochemical assays to detect rat primary antibodies. SignalStain^® Boost IHC Detection Reagent (AP, Rat) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rat IgG. It can be used to visualize targets in both paraffin-embedded and frozen tissue sections and it is compatible with all alkaline phosphatase-based substrates.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

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