Revision 1

#36775Store at -20C

1 Kit

(10 slides)

UniProt ID:

#P11836

Entrez-Gene Id:

#931

Cell Signaling Technology

Orders: 877-616-CELL (2355) orders@cellsignal.com

Support: 877-678-TECH (8324)

Web: info@cellsignal.com cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Item # Volume Reactivity Isotype
CD20 (E7B7T) XP® Rabbit mAb (SignalStar Conjugate 0011) 40100 50 µl H Rabbit IgG
Complementary Oligo (CO-0011-647) 62170 22 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

CD20 (E7B7T) XP® Rabbit mAb (SignalStar Conjugate 0011) recognizes endogenous levels of total CD20 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with human CD20 recombinant protein.

Background

B-lymphocyte antigen CD20 (also known as MS4A1; Membrane-spanning 4-domains subfamily A member 1) is a cell surface phosphoprotein involved in the regulation of B cell activation and proliferation (1,2). It is commonly used as a marker to identify B cells and is expressed throughout B cell development, up until their differentiation into plasma cells. CD20 has no known ligand, and its expression and function are largely conserved between human and mouse (1-3). Evidence suggests that CD20 is necessary for store operated calcium (SOC) entry, which leads to elevated cytoplasmic calcium levels required for B cell activation (4,5). Anti-CD20 antibody immunotherapy depletes B cells by activation of the innate monocytic network and is a common treatment for B cell lymphomas, leukemias, and autoimmune diseases (6).

  1. Stashenko, P. et al. (1980) J Immunol 125, 1678-85.
  2. Tedder, T.F. et al. (1985) J Immunol 135, 973-9.
  3. Tedder, T.F. et al. (1988) J Immunol 141, 4388-94.
  4. Bubien, J.K. et al. (1993) J Cell Biol 121, 1121-32.
  5. Li, H. et al. (2003) J Biol Chem 278, 42427-34.
  6. Uchida, J. et al. (2004) J Exp Med 199, 1659-69.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStar is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 1
#36775

CD20 (E7B7T) & CO-0011-647 SignalStar Oligo-Antibody Pair

Immunohistochemistry Image 1: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded human tonsil using CD20 (E7B7T) & CO-0011-594 SignalStar Oligo-Antibody Pair #54189 (yellow), Phospho-SLP-76 (Ser376) (E3G9U) & CO-0018-488 SignalStar Oligo-Antibody Pair #34567 (green), CD3ε (D7A6E) & CO-0001-488 SignalStar Oligo-Antibody Pair #92856 (pink), CD8α (D8A8Y) & CO-0004-594 SignalStar Oligo-Antibody Pair #19166 (orange), ICOS (D1K2T) & CO-0027-647 SignalStar Oligo-Antibody Pair #24147 (red), Granzyme B (D6E9W) & CO-0009-647 SignalStar Oligo-Antibody Pair #37369 (white), Ki-67 (8D5) & CO-0014-750 SignalStar Oligo-Antibody Pair #56398 (cyan), Pan-Keratin (C11) & CO-0003-750 SignalStar Oligo-Antibody Pair #97227 (magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Immunohistochemistry Image 2: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD20 (E7B7T) & CO-0011-488 SignalStar Oligo-Antibody Pair #77332 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Immunohistochemistry Image 3: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar immunohistochemical analysis of paraffin-embedded human tonsil using CD20 (E7B7T) & CO-0011-594 SignalStar Oligo-Antibody Pair #54189 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Immunohistochemistry Image 4: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD20 (E7B7T) & CO-0011-647 SignalStar Oligo-Antibody Pair #36775 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Immunohistochemistry Image 5: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar immunohistochemical analysis of paraffin-embedded human tonsil using CD20 (E7B7T) & CO-0011-750 SignalStar Oligo-Antibody Pair #75882 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Immunohistochemistry Image 6: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD20 (E7B7T) & CO-0011-647 SignalStar Oligo-Antibody Pair #36775 (left, red) and ProLong Gold Antifade Reagent with DAPI #8961 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human non-small cell lung carcinoma using CD20 (E7B7T) XP® Rabbit mAb #48750 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Immunohistochemistry Image 7: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD20 (E7B7T) & CO-0011-647 SignalStar Oligo-Antibody Pair #36775 (pink), Pan-Keratin (C11) & CO-0003-488 SignalStar Oligo-Antibody Pair #63566 (red), Granzyme B (D6E9W) & CO-0009-594 SignalStar Oligo-Antibody Pair #15194 (orange), CD8α (D8A8Y) & CO-0004-750 SignalStar Oligo-Antibody Pair #62750 (white), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX by Leica Biosystems.
Immunohistochemistry Image 1: CD20 (E7B7T) & CO-0011-647 SignalStar™ Oligo-Antibody Pair Expand Image
SignalStar multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD20 (E7B7T) & CO-0011-594 SignalStar Oligo-Antibody Pair #54189 (yellow), LAG3 (D2G4O) & CO-0026-488 SignalStar Oligo-Antibody Pair #69276 (green), Granzyme B (D6E9W) & CO-0009-647 SignalStar Oligo-Antibody Pair #37369 (red), HLA-DRA (E9R2Q) & CO-0023-750 SignalStar Oligo-Antibody Pair #58446 (cyan), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). All fluorophores have been assigned a pseudocolor, as indicated.