Loading control antibodies are used to determine equal loading as well as the quality of the samples. This is critically important to aid in the interpretation of western blot results. Proteins used as loading controls are typically selected for their ubiquitous expression across all cell lines and tissues as well as their abundance. Selecting the right loading control antibody is determined by the molecular weight of the other targets being studied, subcellular fraction of the sample, and the cell and tissue types being evaluated.
You need to trust that your loading control is sensitive and specific to its target protein. If your loading control is questionable, it can create doubt about all of your results. Use loading controls you trust for the most reliable experimental results.
| Loading Control | Size (kDa) | Recommended for: |
|---|---|---|
| β-Actin | 45 | Whole Cell Lysate |
| GAPDH | 37 | |
| β-Tubulin | 55 | |
| α-Tubulin | 52 | |
| Vinculin | 124 | |
| α-Actinin | 100 | |
| Cyclophilin B | 19 | |
| Loading Control | Size (kDa) | Recommended for: |
| Histone H3 | 17 | Nuclear Fraction |
| HDAC1 | 62 | |
| Loading Control | Size (kDa) | Recommended for: |
| COX IV | 17 | Mitochondrial Fraction |
| VDAC | 32 | |
| SDHA | 70 |
| No. | Name |
|---|---|
| 5142 | Loading Control Antibody Sampler Kit |
| 9774 | Loading Control Antibody Sampler Kit (Mouse) |
| 4670 | Loading Control Antibody Sampler Kit (HRP Conjugate) |
