The accuracy of western blot results relies heavily of the quality of the primary antibody used. CST™ antibodies are validated using multiple experiments.
A demonstration of the perils of deviating from Cell Signaling Technology's recommended protocols.
Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experim
Immunoprecipitation Protocol (For Analysis By Kinase Assay): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation of Denatured Proteins Protocol: easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Troubleshooting Guide for easy to solve high and low background issues.
In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.
Ensure equal sample loading with loading control antibodies from CST.
A table that lists shelf-life expectations for CST products (when stored under the recommended conditions).
Western Immunoblotting Protocol: easy to follow directions describing the step by step experimental procedure.
Western Blotting (Fluorescent) Protocol: easy to follow directions describing the step by step experimental procedure.
Western Blotting-Conformation, LC Specific, Anti-Biotin Secondary Ab: easy to follow directions describing the step by step experimental procedure.
Western Blotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.
Western Blotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.
CST's Western Blotting Protocol shows you the critical experimental steps in western blotting and explains how small changes to the protocol can affect the final results.
Western Blotting troubleshooting guide for easy to solve high and low background issues.
CST's Western Blotting (WB) Troubleshooting Guide Video provides tips to help you diagnose experimental issues and offers recommendations to solve the problems.
Short videos from CST highlighting our optimized western blotting protocol and troubleshooting guide to diagnose issues and recommend solutions.
