Three major categories of methods exist for detection in ELISA: colorimetric, fluorescent, and chemiluminescent. The type of substrate used depends on the needed assay sensitivity and on the signal-detection limits of the instrument used to read the plat
The following three types of analysis are used to analyze results from ELISA assays: qualitative, semiquantitative, and quantitative.
ELISA Protocol for PathScan® Stress and Apoptosis Signaling Antibody Array Kit (Chemiluminescent Readout) #12856: easy to follow directions describing the step by step experimental procedure.
ELISA Protocol for PathScan® Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout) #12923: easy to follow directions describing the step by step experimental procedure.
CST ELISA kits and reagents let you measure key cellular signaling nodes and other cellular target proteins using a wide selection of sandwich antibody assays.
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
A scientific resource for the ENTH protein domain containing information on structure, function, and domain binding to phospholipids during endocytosis.
The Corporate Social Responsibility of Cell Signaling Technology, Inc.
CST supports environmental projects and science education by providing small grants to local organizations.
Enzyme Immuno Assay Kit Protocol: easy to follow directions describing the step by step experimental procedure.
In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.
Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.
Compare Cell Signaling Technology's Erk antibodies against one another to determine optimal applications
Phospho-Erk product lists, product citation lists, product selection tools, comparison tables and educational resources for MAPK signaling from Cell Signaling Technology.
A scientific resource for the EVH1 protein domain containing information on structure, function, and binding to a proline-rich motif to regulate actin dynamics.
Case Study 4: Exceptional Performance of XP Monoclonal Antibodies in All Applications pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Case Study 3: Exceptional Reproducibility of XP® Monoclonal Antibodies in All Applications pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Case Study 2: Exceptional Sensitivity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
A scientific resource for the F-Box protein domain containing information on structure, function, and domain binding to ubiquitin in protein degradation pathways.
FastScan ELISA kits detect key signaling proteins using a solution-based assay format and are validated to ensure reproducible results across lots and users.
A scientific resource for the FERM protein domain containing information on structure, function, and domain binding in cytoskeleton-associated proteins.
A scientific resource for the FF protein domain containing information on structure, function, and binding to phospho-serine motifs.
A scientific resource for the FH2 protein domain containing information on structure, function, and domain binding to actin.
A scientific resource for the FHA protein domain containing information on structure, function, and binding to phospho-serine and phospho-threonine motifs.
Use the lookup tool to find the CST equivalent for your SCBT product.
Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.
Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Live Cell Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.
Flow cytometry resources and products from CST Cell Signaling Technology
Technical support questions and issues for flow cytometry-validated antibodies and products from CST Cell Signaling Technology
Troubleshooting tips for successfully performing Intracellular Flow Cytometry using CST recommended protocols and antibodies validated for Flow.
Flow Cytometry, Methanol Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry, Triton™ X-100 Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
Protocol for Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification
Western blotting using fluorescent-conjugated secondary antibodies - accurate quantification and two-color multiplexing using primary antibodies from different species.
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.