Three major categories of methods exist for detection in ELISA: colorimetric, fluorescent, and chemiluminescent. The type of substrate used depends on the needed assay sensitivity and on the signal-detection limits of the instrument used to read the plat
The following three types of analysis are used to analyze results from ELISA assays: qualitative, semiquantitative, and quantitative.
ELISA Protocol for PathScan® Stress and Apoptosis Signaling Antibody Array Kit (Chemiluminescent Readout) #12856: easy to follow directions describing the step by step experimental procedure.
ELISA Protocol for PathScan® Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout) #12923: easy to follow directions describing the step by step experimental procedure.
CST ELISA kits and reagents let you measure key cellular signaling nodes and other cellular target proteins using a wide selection of sandwich antibody assays.
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
A scientific resource for the ENTH protein domain containing information on structure, function, and domain binding to phospholipids during endocytosis.
The Corporate Social Responsibility of Cell Signaling Technology, Inc.
CST supports environmental projects and science education by providing small grants to local organizations.
Enzyme Immuno Assay Kit Protocol: easy to follow directions describing the step by step experimental procedure.
In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.
Epigenetics
(Pathway Diagram)
Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.
Erk Antibody Comparison Table
Compare Cell Signaling Technology's Erk antibodies against one another to determine optimal applications
Erk Signaling
(Product Type Overviews)
Phospho-Erk product lists, product citation lists, product selection tools, comparison tables and educational resources for MAPK signaling from Cell Signaling Technology.
EVH1 Protein Domain
(Protein Domains)
A scientific resource for the EVH1 protein domain containing information on structure, function, and binding to a proline-rich motif to regulate actin dynamics.
Exceptional Performance of XP® Monoclonal Antibodies
(Product Type Overviews)
Case Study 4: Exceptional Performance of XP Monoclonal Antibodies in All Applications pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Exceptional Reproducibility of XP® Monoclonal Antibodies
(Product Type Overviews)
Case Study 3: Exceptional Reproducibility of XP® Monoclonal Antibodies in All Applications pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Exceptional Sensitivity of XP® Monoclonal Antibodies
(Product Type Overviews)
Case Study 2: Exceptional Sensitivity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
Exceptional Specificity of XP® Monoclonal Antibodies
(Product Type Overviews)
Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.
F-Box Protein Domain
(Protein Domains)
A scientific resource for the F-Box protein domain containing information on structure, function, and domain binding to ubiquitin in protein degradation pathways.
FastScan™ ELISA (Enzyme-Linked Immunosorbent Assay) Kits
FastScan ELISA kits detect key signaling proteins using a solution-based assay format and are validated to ensure reproducible results across lots and users.
FERM Protein Domain
(Protein Domains)
A scientific resource for the FERM protein domain containing information on structure, function, and domain binding in cytoskeleton-associated proteins.
FF Protein Domain
(Protein Domains)
A scientific resource for the FF protein domain containing information on structure, function, and binding to phospho-serine motifs.
FH2 Protein Domain
(Protein Domains)
A scientific resource for the FH2 protein domain containing information on structure, function, and domain binding to actin.
FHA Protein Domain
(Protein Domains)
A scientific resource for the FHA protein domain containing information on structure, function, and binding to phospho-serine and phospho-threonine motifs.
Find alternatives to Santa Cruz antibodies
Use the lookup tool to find the CST equivalent for your SCBT product.
Flow Cytometry (F) Protocol for the Detection of Transcription Factors
(Protocols)
Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.
Flow Cytometry BrdU Modified Protocol
(Protocols)
Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Live Cell Protocol
(Protocols)
Flow Cytometry Live Cell Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol (Flow)
(Protocols)
Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for Extracellular Staining Using Conjugated Secondary Antibodies
(Protocols)
Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC)
(Protocols)
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells
(Protocols)
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Resource Center
(Application Overviews)
Flow cytometry resources and products from CST Cell Signaling Technology
Flow Cytometry Technical Support
(Protocols)
Technical support questions and issues for flow cytometry-validated antibodies and products from CST Cell Signaling Technology
Flow Cytometry Troubleshooting Guide
(Protocols)
Troubleshooting tips for successfully performing Intracellular Flow Cytometry using CST recommended protocols and antibodies validated for Flow.
Flow Cytometry, Methanol Permeabilization Protocol
(Protocols)
Flow Cytometry, Methanol Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
Flow Cytometry, Triton™ X-100 Permeabilization Protocol
(Protocols)
Flow Cytometry, Triton™ X-100 Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification Protocol
(Protocols)
Protocol for Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification
Fluorescent Western Blotting Resources
Western blotting using fluorescent-conjugated secondary antibodies - accurate quantification and two-color multiplexing using primary antibodies from different species.
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA)
(Protocols)
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk)
(Protocols)
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.
Frequently Asked Questions
Frequently asked questions using our products while doing your research
FYVE Protein Domain
(Protein Domains)
A scientific resource for the FYVE protein domain containing information on structure, function, and binding to phospholipids.
GAT Protein Domain
(Protein Domains)
A scientific resource for the GAT protein domain containing information on structure, function, and domain binding to ubiquitin and GTP-bound ARF.
GEL Protein Domain
(Protein Domains)
A scientific resource for the GEL protein domain containing information on structure, function, and domain binding to actin in cytoskeletal regulation.
A scientific resource for the GLUE protein domain containing information on structure, function, and binding to phospholipids in the regulation of vesicle trafficking.
GPCR, Calcium, cAMP
(Pathway Diagram)
Overview of GPCR, Calcium, cAMP Signaling Resources, interactive pathway diagrams, and other technical resources.
GRAM Protein Domain
(Protein Domains)
A scientific resource for the GRAM protein domain containing information on structure, function, and phospholipid binding during vesicle trafficking.
Green Committee
Cell Signaling Technology supports environmental initiatives through an internal group who review ways for CST to be sustainable.
Greenhouse Gas and Energy Management
CST has an energy management plan to reduce our use of resources.
Greenhouse Gas Management
CST is committed to following the Paris Climate Agreement.
GRIP Protein Domain
(Protein Domains)
A scientific resource for the GRIP protein domain containing information on structure, function, and domain binding to ARF family GTPases during vesicle trafficking.
GYF Protein Domain
(Protein Domains)
A scientific resource for the GYF protein domain containing information on structure, function, and domain binding to proline-rich motifs.
HEAT Protein Domain
(Protein Domains)
A scientific resource for the HEAT protein domain containing information on structure, function, and domain binding.
HECT Protein Domain
(Protein Domains)
A scientific resource for the HECT protein domain containing information on structure, function, and domain binding in ubiquitin-mediated protein degradation.
High Content Screening
(Application Overviews)
Validated antibodies for high content screening from CST Cell Signaling Technology
G-Protein-Coupled Receptors Signaling to MAPK/Erk
(Pathway Diagram)
G1/S Checkpoint
(Pathway Diagram)