Histone Modification Table
(Table Resources)
The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.
How Does PTMScan® Direct Proteomic Service Work?
(Proteomics Overviews)
Overview of methodology and client deliverables for the PTMScan Direct Proteomic Service offered by Cell Signaling Technology.
How to Analyze Multiple Markers in Tissue Samples Using Multiplex IHC
Multiplex immunohistochemistry (mIHC): Perform in depth tissue analysis on a single slide to understand protein co-localization and spatial context
How to Choose Enzymatic or Sonication Protocol for ChIP
This webinar will focus on the importance of the immune regulatory context of the TME
How We Select Our Antibody Targets
(Process Explanations)
How CST responds to customer feedback as it relates to development of the product portfolio.
HRP Chemiluminescence ELISA-P
(Protocols)
HRP Chemiluminescence ELISA-Pl: easy to follow directions describing the step by step experimental procedure.
Human Immune Cell Marker Guide
(Pathway Diagram)
This interactive guide shows the key cell and functional state markers for tumor-infiltrating immune cells in humans.Web Location: Immunology and Inflammation
Human Oncogenes & Tumor Suppressor Genes
(Table Resources)
Summary of human oncogenes and tumor suppressor genes sorted by signaling pathway.
Human Protein Kinases Overview
(Kinome Diagram)
Interactive kinome diagram of the human protein kinases.
Human Tyrosine Protein Kinases
(Kinome Diagram)
A kinome diagram showing the human tyrosine kinase group. This group of protein kinases comprises receptor and non-receptor tyrosine kinases.
IMAC Service
(Proteomics Overviews)
Quantitating Ser/Thr phosphorylation by Immobilized Metal Affinity Chromatography enrichment and analysis identifies thousands of sites with minimal sample.
Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting)
(Protocols)
Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experim
Immuno-Parallel Reaction Monitoring (iPRM)
(Proteomics Overviews)
iPRM is a multiplexed LC/MS assay service that combines the specificity of mass spectrometry, the throughput of an immunoassay, and the power of multiplexing to accelerate the time-to-market of your drug and/or diagnostic test.
Immunofluorescence (IF) Resource Center
(Application Overviews)
Immunofluorescence-validated antibodies from CST Cell Signaling Technology
Immunofluorescence (IF) Troubleshooting Guide
A helpful guide to help answer questions and troubleshoot common problems encountered in immunofluorescence.
Immunofluorescence Protocol for Biotin-Conjugated Antibodies
(Protocols)
Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol for Cell-based Assays Requiring Methanol Fixation
(Protocols)
Immunofluorescence Protocol wit Methanol Fixation (IF Methanol-fixed): easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol for Methanol Fixation (IF Methanol-fixed): easy to follow directions describin
Immunofluorescence Protocol for Labeling with BrdU Antibody
(Protocols)
Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization
(Protocols)
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experim
Immunofluorescence Protocol with Formaldehyde Fixation
(Protocols)
Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Gelatin Blocking Step
(Protocols)
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimenta
Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer
(Protocols)
Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by st
Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm)
(Protocols)
Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step exp
Immunofluorescence Protocol with n-Octyl-Glucoside PermeabilizationTechnology
(Protocols)
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing th
Immunofluorescence Protocol with Saponin Permeabilization
(Protocols)
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol without Permeabilization
(Protocols)
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol – Specific for Product: 2187
(Protocols)
Immunofluorescence Protocol – Specific for Product: 2187: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Technical Support
(Protocols)
Immunofluorescence Technical Support | CST Cell Signaling Technology
Immunohistochemical Analysis
Learn how to best interpret IHC staining and understand experiment results accurately.
Immunohistochemistry (IHC) Troubleshooting Guide & the Importance of Using Controls
(Protocols)
Learn how to best interpret IHC staining and understand experiment results accurately.
Immunohistochemistry Leica® BOND™ Protocol
(Protocols)
Immunohistochemistry Leica® Bond™ Protocol: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Frozen)
(Protocols)
Immunohistochemistry Protocol (Frozen): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060
(Protocols)
Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin)
(Protocols)
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3287
(Protocols)
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3625
(Protocols)
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3633
(Protocols)
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific for Product: 9319
(Protocols)
Immunohistochemistry Protocol (Paraffin) - Specific for Product: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197
(Protocols)
Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol - Frozen for SignalStain® Boost Detection Reagent
(Protocols)
Immunohistochemistry Protocol - Frozen for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol - Paraffin for SignalStain® Boost Detection Reagent
(Protocols)
Immunohistochemistry Protocol – Paraffin for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol - Paraffin for Boost Detection Reagent: easy to follow directions describing
Immunohistochemistry Protocol - Specific For Product: 12692
(Protocols)
Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.
Immunohistochemistry Technical Support
Cell Signaling Technology IHC technical support contact information and outline of requested information that our technical support specialists will need to assist you.
Immunohistochemistry Tips and Techniques
Learn how your Immunohistochemistry protocol affects your results.
Immunology/Inflammation
(Pathway Diagram)
Expert-reviewed interactive pathways providing current overviews of NF-kB and Toll-like Receptor (TLR) signaling, as well as links to products from Cell Signaling Technology.
Immunoprecipitation Protocol (For Analysis By Kinase Assay)
(Protocols)
Immunoprecipitation Protocol (For Analysis By Kinase Assay): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Analysis By Western Immunoblotting)
(Protocols)
Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Native Protein)
(Protocols)
Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol / (For Denatured Proteins) (IP / Denatured Protein)
(Protocols)
Immunoprecipitation of Denatured Proteins Protocol: easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Immunoblotting)
(Protocols)
Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Troubleshooting Guide
(Protocols)
Immunoprecipitation Troubleshooting Guide for easy to solve high and low background issues.
In-Cell Western Protocol
(Protocols)
In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.
In-Cell Western™
(Application Overviews)
In-cell Western: a simple method for quantification of intracellular signaling in whole cells. Data and links for tech support.
Interactive Human Kinome
(Kinome Diagram)
Searchable Human Kinome showing the AGC, Tyrosine Kinases, TKL, CAMK, STE, CMGC, CK1 and atypical kinase groups.
IQ Protein Domain
(Protein Domains)
A scientific resource for the IQ protein domain containing information on structure, function, and domain binding to calmodulin.
Jak and Cytokine Receptor Mutants in Cancer Table
(Table Resources)
Jak and cytokine receptor mutations found in various cancers, along with the corresponding PubMed reference(s).
Jak/Stat Utilization Table
(Table Resources)
The Jak/Stat Utilization Table displays the combinatorial use of tyrosine kinases and Stat proteins in cytokine/growth factor signaling.
Key Signaling Networks in Cancer
Cancer signaling pathways and research resources, including activation state antibodies and proteomic tools to discover post-translational modifications
Kinase Signaling
(Pathway Diagram)
Expert-reviewed interactive pathway providing a current overview of ErbB/HER Signaling, a pathway description, select PubMed references, and links to PhosphoSitePlus.
Kinase-Disease Associations
(Table Resources)
Kinase-disease associations table showing kinases, kinase groups, disease types, and the molecular biology basis for disease.
KinomeView® Profiling
(Proteomics Overviews)
Information on KinomeView western blotting service offered by Cell Signaling Technology that uses Phospho-Motif Antibodies to provide a kinome-wide view of cellular phosphorylation.
KinomeView® Protocol
(Protocols)
KinomeView® Protocol: easy to follow directions describing the step by step experimental procedure.
LC/MS Proteomics Resource Center
(Proteomics Overviews)
Overview of PTMScan technology for proteomics from CST. Antibody enrichment of PTM peptides combined with mass spectrometry.
Learn & Support
Find educational resources related to science or get technical support help with a product.
Learning Lab LIVE! Q&A with IHC Expert Chris Grange
This webinar recording focuses on how to optimize two steps in the immunohistochemistry protocol. Antigen retrieval is required to reverse chemical crosslinks that can prevent antibodies from accessing their epitopes; the conditions of the primary antibo
LEED (Leadership in Energy and Environmental Design)
The home office of CST is a LEED certified building.
LIM Protein Domain
(Protein Domains)
A scientific resource for the LIM protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.
Loading Control Antibodies
(Product Type Overviews)
Ensure equal sample loading with loading control antibodies from CST.
LRR Protein Domain
(Protein Domains)
A scientific resource for the LRR protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.
m6A RNA Regulatory Diagram
(Pathway Diagram)
Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development
MAP Kinase Signaling Resources
(Pathway Diagram)
Overview of MAP Kinase signaling pathways, antibodies and related reagents, interactive pathway diagrams, and other technical resources.
Mastering Phospho-Flow: Immune Cell Activation
To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal.
MBT Protein Repeat
(Protein Domains)
A scientific resource for the MBT protein domain containing information on structure, function, and binding to methyl-lysine motifs during chromatin regulation.
Mechanisms of Fibrosis
(Pathway Diagram)
Expert-reviewed diagram providing a current overview of the Mechanisms of Fibrosis pathway with references to its role in adhesion
Membrane-Enriched Total Proteome Service
Identify and quantify membrane proteins in a total proteome study for a more complete view of proteins on the cell surface.
Metabolic Changes in Cancer: The Warburg Effect
CST sponsored Science Webinar Series: Metabolic Changes in Cancer: April 19, 2012. Hear the webinar (3 speakers), download slides and transcripts.
MH2 Protein Domain
(Protein Domains)
A scientific resource for the MH2 protein domain containing information on structure, function, and domain binding to phospho-serine motifs during TGF-beta signaling.
MIU Protein Domain
(Protein Domains)
A scientific resource for the MIU protein domain containing information on structure, function, and domain binding to ubiquitin during vesicle trafficking.
Motif Antibodies for PTMScan® Discovery Services
(Proteomics Overviews)
A listing of PTMScan Motif Antibodies and Kits, along with case study data for each, for customers who purchase a license and would like to perform PTMScan Proteomics in-house.
Mouse Immune Cell Marker Guide
(Pathway Diagram)
This interactive guide shows the most important cell and functional state markers for tumor-infiltrating immune cells in mice.
Multiplex IHC
Fluorescent multiplex IHC (mIHC) with tyramide signal amplification enables simultaneous detection of multiple proteins in formalin-fixed paraffin-embedded (FFPE) tissue sections in a sequential fashion.
Multiplex Oligos for Illumina® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN)
(Protocols)
Multiplex Oligos for Illumina® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.
Multiplex Oligos for Illumina® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN)
(Protocols)
Multiplex Oligos for Illumina® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.a
Neural and Glial Cell Markers
There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.
Neuroinflammation in Neurodegenerative Diseases
Neuroinflammation is the activation of an immune response in the CNS by the microglia and astrocytes. While not linked mechanistically to neurodegenerative diseases, neuroinflammation is associated with the progression of Alzheimer's disease, Parkinson's
Neuronal and Glial Cell Markers
(Pathway Diagram)
Expert-reviewed interactive diagrams providing current overviews of neuronal and glial cell markers, as well as links to products from CST.
Neuroscience
(Pathway Diagram)
Expert-reviewed interactive neuroscience pathways providing current overviews of Parkinsons and Alzheimers Disease signaling, as well as links to products from CST.
Neurotransmitters, Receptors, and Transporters
Modulation of CNS function occurs through synaptic neurotransmission, which is the signaling from the axon terminal of one neuron to the dendrites of another via molecules called neurotransmitters.
Nuclear Permeabilization Staining
(Protocols)
Nuclear Permeabilization Staining: easy to follow directions describing the step by step experimental procedure.
Nuclear Receptors
(Pathway Diagram)
Expert-reviewed interactive pathway providing a current overview of Nuclear Receptor Signaling, a pathway description, select PubMed references, and links to PhosphoSitePlus.
NZF Protein Domain
(Protein Domains)
A scientific resource for the NZF protein domain containing information on structure, function, and domain binding to ubiquitin.
Optimizing the ELISA (Enzyme-Linked Immunosorbent Assay) Test
Optimization is critical to obtaining reliable ELISA results. The design and development of an ELISA assay depends on the various components, including capture antibody, blocking buffer, diluent, sample concentration, and detection antibody
Organelle Markers
(Application Overviews)
Organelle markers for immunofluorescence analysis from CST Cell Signaling Technology
Other Protein Kinases Group
(Kinome Diagram)
Kinome diagram showing "other" group kinases.
Our Approach/Process
Learn more about our approach to science and process for creating the best antibodies in the industry
Overview of Chromatin Immunoprecipitation (ChIP)
(Application Overviews)
Chromatin immunoprecipitation, or ChIP, is a technique that uses antibodies to isolate specific DNA-binding proteins along with the bound DNA fragments from cells and tissues.
Overview of Chromatin IP Assay Methodology
(Application Overviews)
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique to probe protein-DNA interactions within the natural chromatin context of the cell.
Overview of Immunostaining
Immunofluorescence (IF) techniques combine antibodies and fluorescence imaging to visualize biomolecules within fixed cell or tissue samples. This page answers common protocol questions to help your IF experiment be successful.
Overview of Metabolism and Metabolic Disorders
Metabolism defines all of the chemical changes or processes within cells, tissues, and organisms that sustain cellular homeostasis.
Hippo Signaling
(Pathway Diagram)
Inflammasome Signaling
(Pathway Diagram)
NF-κB Signaling
(Pathway Diagram)
p38 MAPK Signaling
(Pathway Diagram)