Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.
Frequently asked questions using our products while doing your research
A scientific resource for the FYVE protein domain containing information on structure, function, and binding to phospholipids.
GAT Protein Domain
(Protein Domains)
A scientific resource for the GAT protein domain containing information on structure, function, and domain binding to ubiquitin and GTP-bound ARF.
GEL Protein Domain
(Protein Domains)
A scientific resource for the GEL protein domain containing information on structure, function, and domain binding to actin in cytoskeletal regulation.
A scientific resource for the GLUE protein domain containing information on structure, function, and binding to phospholipids in the regulation of vesicle trafficking.
GPCR, Calcium, cAMP
(Pathway Diagram)
Overview of GPCR, Calcium, cAMP Signaling Resources, interactive pathway diagrams, and other technical resources.
GRAM Protein Domain
(Protein Domains)
A scientific resource for the GRAM protein domain containing information on structure, function, and phospholipid binding during vesicle trafficking.
Green Committee
Cell Signaling Technology supports environmental initiatives through an internal group who review ways for CST to be sustainable.
Greenhouse Gas and Energy Management
CST has an energy management plan to reduce our use of resources.
Greenhouse Gas Management
CST is committed to following the Paris Climate Agreement.
GRIP Protein Domain
(Protein Domains)
A scientific resource for the GRIP protein domain containing information on structure, function, and domain binding to ARF family GTPases during vesicle trafficking.
GYF Protein Domain
(Protein Domains)
A scientific resource for the GYF protein domain containing information on structure, function, and domain binding to proline-rich motifs.
HEAT Protein Domain
(Protein Domains)
A scientific resource for the HEAT protein domain containing information on structure, function, and domain binding.
HECT Protein Domain
(Protein Domains)
A scientific resource for the HECT protein domain containing information on structure, function, and domain binding in ubiquitin-mediated protein degradation.
High Content Screening
(Application Overviews)
Validated antibodies for high content screening from CST Cell Signaling Technology
Histone Modification Table
(Table Resources)
The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.
How Does PTMScan® Direct Proteomic Service Work?
(Proteomics Overviews)
Overview of methodology and client deliverables for the PTMScan Direct Proteomic Service offered by Cell Signaling Technology.
How to Analyze Multiple Markers in Tissue Samples Using Multiplex IHC
Multiplex immunohistochemistry (mIHC): Perform in depth tissue analysis on a single slide to understand protein co-localization and spatial context
How to Choose Enzymatic or Sonication Protocol for ChIP
This webinar will focus on the importance of the immune regulatory context of the TME
How We Select Our Antibody Targets
(Process Explanations)
How CST responds to customer feedback as it relates to development of the product portfolio.
HRP Chemiluminescence ELISA-P
(Protocols)
HRP Chemiluminescence ELISA-Pl: easy to follow directions describing the step by step experimental procedure.
Human Immune Cell Marker Guide
(Pathway Diagram)
This interactive guide shows the key cell and functional state markers for tumor-infiltrating immune cells in humans.Web Location: Immunology and Inflammation
Human Oncogenes & Tumor Suppressor Genes
(Table Resources)
Summary of human oncogenes and tumor suppressor genes sorted by signaling pathway.
Human Protein Kinases Overview
(Kinome Diagram)
Interactive kinome diagram of the human protein kinases.
Human Tyrosine Protein Kinases
(Kinome Diagram)
A kinome diagram showing the human tyrosine kinase group. This group of protein kinases comprises receptor and non-receptor tyrosine kinases.
IMAC Service
(Proteomics Overviews)
Quantitating Ser/Thr phosphorylation by Immobilized Metal Affinity Chromatography enrichment and analysis identifies thousands of sites with minimal sample.
Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting)
(Protocols)
Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experim
Immuno-Parallel Reaction Monitoring (iPRM)
(Proteomics Overviews)
iPRM is a multiplexed LC/MS assay service that combines the specificity of mass spectrometry, the throughput of an immunoassay, and the power of multiplexing to accelerate the time-to-market of your drug and/or diagnostic test.
Immunofluorescence (IF) Resource Center
(Application Overviews)
Immunofluorescence-validated antibodies from CST Cell Signaling Technology
Immunofluorescence (IF) Troubleshooting Guide
A helpful guide to help answer questions and troubleshoot common problems encountered in immunofluorescence.
Immunofluorescence Protocol for Biotin-Conjugated Antibodies
(Protocols)
Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol for Cell-based Assays Requiring Methanol Fixation
(Protocols)
Immunofluorescence Protocol wit Methanol Fixation (IF Methanol-fixed): easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol for Methanol Fixation (IF Methanol-fixed): easy to follow directions describin
Immunofluorescence Protocol for Labeling with BrdU Antibody
(Protocols)
Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization
(Protocols)
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experim
Immunofluorescence Protocol with Formaldehyde Fixation
(Protocols)
Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Gelatin Blocking Step
(Protocols)
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimenta
Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer
(Protocols)
Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by st
Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm)
(Protocols)
Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step exp
Immunofluorescence Protocol with n-Octyl-Glucoside PermeabilizationTechnology
(Protocols)
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing th
Immunofluorescence Protocol with Saponin Permeabilization
(Protocols)
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol without Permeabilization
(Protocols)
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol – Specific for Product: 2187
(Protocols)
Immunofluorescence Protocol – Specific for Product: 2187: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Technical Support
(Protocols)
Immunofluorescence Technical Support | CST Cell Signaling Technology
Immunohistochemical Analysis
Learn how to best interpret IHC staining and understand experiment results accurately.
Immunohistochemistry (IHC) Troubleshooting Guide & the Importance of Using Controls
(Protocols)
Learn how to best interpret IHC staining and understand experiment results accurately.
Immunohistochemistry Leica® BOND™ Protocol
(Protocols)
Immunohistochemistry Leica® Bond™ Protocol: easy to follow directions describing the step by step experimental procedure.
G-Protein-Coupled Receptors Signaling to MAPK/Erk
(Pathway Diagram)
G1/S Checkpoint
(Pathway Diagram)
Hippo Signaling
(Pathway Diagram)