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Immunohistochemistry Protocol (Frozen) (Protocols)

Immunohistochemistry Protocol (Frozen): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060 (Protocols)

Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060: easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3287 (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3625 (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3633 (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific for Product: 9319 (Protocols)

Immunohistochemistry Protocol (Paraffin) - Specific for Product: easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197 (Protocols)

Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197: easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol - Frozen for SignalStain® Boost Detection Reagent (Protocols)

Immunohistochemistry Protocol - Frozen for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol - Paraffin for SignalStain® Boost Detection Reagent (Protocols)

Immunohistochemistry Protocol – Paraffin for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol - Paraffin for Boost Detection Reagent: easy to follow directions describing

Immunohistochemistry Protocol - Specific For Product: 12692 (Protocols)

Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.

Immunohistochemistry Technical Support

Cell Signaling Technology IHC technical support contact information and outline of requested information that our technical support specialists will need to assist you.

Immunohistochemistry Tips and Techniques

Learn how your Immunohistochemistry protocol affects your results.

Immunology/Inflammation (Pathway Diagram)

Expert-reviewed interactive pathways providing current overviews of NF-kB and Toll-like Receptor (TLR) signaling, as well as links to products from Cell Signaling Technology.

Immunoprecipitation Protocol (For Analysis By Kinase Assay) (Protocols)

Immunoprecipitation Protocol (For Analysis By Kinase Assay): easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) (Protocols)

Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol (For Native Protein) (Protocols)

Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol / (For Denatured Proteins) (IP / Denatured Protein) (Protocols)

Immunoprecipitation of Denatured Proteins Protocol: easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Immunoblotting) (Protocols)

Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Troubleshooting Guide (Protocols)

Immunoprecipitation Troubleshooting Guide for easy to solve high and low background issues.

In-Cell Western Protocol (Protocols)

In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.

In-Cell Western™ (Application Overviews)

In-cell Western: a simple method for quantification of intracellular signaling in whole cells. Data and links for tech support.

Interactive Human Kinome (Kinome Diagram)

Searchable Human Kinome showing the AGC, Tyrosine Kinases, TKL, CAMK, STE, CMGC, CK1 and atypical kinase groups.

IQ Protein Domain (Protein Domains)

A scientific resource for the IQ protein domain containing information on structure, function, and domain binding to calmodulin.

Jak and Cytokine Receptor Mutants in Cancer Table (Table Resources)

Jak and cytokine receptor mutations found in various cancers, along with the corresponding PubMed reference(s).

Jak/Stat Utilization Table (Table Resources)

The Jak/Stat Utilization Table displays the combinatorial use of tyrosine kinases and Stat proteins in cytokine/growth factor signaling.

Key Signaling Networks in Cancer

Cancer signaling pathways and research resources, including activation state antibodies and proteomic tools to discover post-translational modifications

Kinase Signaling (Pathway Diagram)

Expert-reviewed interactive pathway providing a current overview of ErbB/HER Signaling, a pathway description, select PubMed references, and links to PhosphoSitePlus.

Kinase-Disease Associations (Table Resources)

Kinase-disease associations table showing kinases, kinase groups, disease types, and the molecular biology basis for disease.

KinomeView® Profiling (Proteomics Overviews)

Information on KinomeView western blotting service offered by Cell Signaling Technology that uses Phospho-Motif Antibodies to provide a kinome-wide view of cellular phosphorylation.

KinomeView® Protocol (Protocols)

KinomeView® Protocol: easy to follow directions describing the step by step experimental procedure.

LC/MS Proteomics Resource Center (Proteomics Overviews)

Overview of PTMScan technology for proteomics from CST. Antibody enrichment of PTM peptides combined with mass spectrometry.

Learn & Support

Find educational resources related to science or get technical support help with a product.

Learning Lab LIVE! Q&A with IHC Expert Chris Grange

This webinar recording focuses on how to optimize two steps in the immunohistochemistry protocol. Antigen retrieval is required to reverse chemical crosslinks that can prevent antibodies from accessing their epitopes; the conditions of the primary antibo

LEED (Leadership in Energy and Environmental Design)

The home office of CST is a LEED certified building.

LIM Protein Domain (Protein Domains)

A scientific resource for the LIM protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.

Loading Control Antibodies (Product Type Overviews)

Ensure equal sample loading with loading control antibodies from CST.

LRR Protein Domain (Protein Domains)

A scientific resource for the LRR protein domain containing information on structure, function, and domain binding to mediate protein-protein interactions.

m6A RNA Regulatory Diagram (Pathway Diagram)

Expert-reviewed diagram providing a current overview of the m6A RNA pathway with references to its role in tumor development

MAP Kinase Signaling Resources (Pathway Diagram)

Overview of MAP Kinase signaling pathways, antibodies and related reagents, interactive pathway diagrams, and other technical resources.

Mastering Phospho-Flow: Immune Cell Activation

To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal.

MBT Protein Repeat (Protein Domains)

A scientific resource for the MBT protein domain containing information on structure, function, and binding to methyl-lysine motifs during chromatin regulation.

Mechanisms of Fibrosis (Pathway Diagram)

Expert-reviewed diagram providing a current overview of the Mechanisms of Fibrosis pathway with references to its role in adhesion

Membrane-Enriched Total Proteome Service

Identify and quantify membrane proteins in a total proteome study for a more complete view of proteins on the cell surface.

Metabolic Changes in Cancer: The Warburg Effect

CST sponsored Science Webinar Series: Metabolic Changes in Cancer: April 19, 2012. Hear the webinar (3 speakers), download slides and transcripts.

MH2 Protein Domain (Protein Domains)

A scientific resource for the MH2 protein domain containing information on structure, function, and domain binding to phospho-serine motifs during TGF-beta signaling.

MIU Protein Domain (Protein Domains)

A scientific resource for the MIU protein domain containing information on structure, function, and domain binding to ubiquitin during vesicle trafficking.

Motif Antibodies for PTMScan® Discovery Services (Proteomics Overviews)

A listing of PTMScan Motif Antibodies and Kits, along with case study data for each, for customers who purchase a license and would like to perform PTMScan Proteomics in-house.

Mouse Immune Cell Marker Guide (Pathway Diagram)

This interactive guide shows the most important cell and functional state markers for tumor-infiltrating immune cells in mice.

Multiplex IHC

Fluorescent multiplex IHC (mIHC) with tyramide signal amplification enables simultaneous detection of multiple proteins in formalin-fixed paraffin-embedded (FFPE) tissue sections in a sequential fashion.

Multiplex Oligos for Illumina® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN) (Protocols)

Multiplex Oligos for Illumina® Protocol (Dual Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.

Multiplex Oligos for Illumina® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN) (Protocols)

Multiplex Oligos for Illumina® Protocol (Single Index Primers) (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.a

Neural and Glial Cell Markers

There are a number of markers that can be used to distinguish the many cell types of the central and peripheral nervous system during development, adult neurogenesis, and the pathogenesis of neurodegenerative disorders.

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