Product Results

Customized and Carrier-Free Formulations

Customized Products and Services

CST services combine CST's high-quality antibodies with expert scientific support.

Customized Proteomics Solutions for Expedited Drug Discovery

CUT&RUN Frequently Asked Questions (Application Overviews)

CUT&RUN Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.

CUT&RUN Kit Protocol (Protocols)

CUT&RUN Protocol: A Visual Guide

CUT&RUN Troubleshooting Guide (Protocols)

CUT&RUN: A New Way to Analyze Epigenetic Marks and Mechanisms

Cytokine Receptor Complexes and Activation

3D animated video tutorial illustrating IL-6 eceptor complex formation and activation.

Cytoskeletal Regulation and Vesicle Trafficking (Pathway Diagram)

Expert-reviewed interactive signaling pathways providing current overviews of the regulation of actin and microtubule dynamics, as well as links to products from CST.

Death Protein Domain (Protein Domains)

A scientific resource for the death protein domain containing information on structure, function, and domain binding during apoptosis.

Death Receptor Signaling (Pathway Diagram)

Deciphering CAR-T Cells: Exploring Functional Mechanisms to Drive Next-Generation Immunotherapy

Deciphering epigenetic regulation of the immune response in cancer

Deciphering Epitranscriptomics: The importance of RNA modifications and RNA-binding proteins in disease

Uncovering the mechanisms underlying RNA modifications and RBP function are emerging frontiers in medicine that take the study of gene regulation a step beyond epigenetics

Deciphering Fibrosis: Drivers of Fibrotic Disease in the Liver and Heart

Understanding the underlying biology of fibrosis is critical for the diagnosis, treatment, and management of cardiac fibrosis, nonalcoholic steatohepatitis (NASH), and other fibrotic diseases

Deciphering Immuno-Oncology: Targeting Cellular Mechanisms of the Tumor Immune Response

Deciphering the cell cycle: The role of cell cycle control in cancer

Deciphering the Role of RNA-Binding Proteins in Neurodevelopment and Disease

DED Protein Domain (Protein Domains)

A scientific resource for the DED protein domain containing information on structure, function, and domain binding during apoptosis.

Detecting CAR Expression with Versatile, Innovative Antibodies

Developmental Biology Signaling (Pathway Diagram)

Overview of stem cell markers, lineage markers, development and differentiation signaling networks, antibodies and related reagents, interactive pathway diagrams, and technical resources.

DH Protein Domain (Protein Domains)

A scientific resource for the DH protein domain containing information on structure, function, and domain binding during Rho GTPase activation.

Diffuse Intrinsic Pontine Glioma (DIPG)

Digestion of CUT&RUN Input DNA by MNase (Protocols)

Diversity in Science and STEM Scholarships

DNA Damage Response (Pathway Diagram)

DNA Library Prep for CUT&RUN: A Visual Guide

DNA Library Prep Kit for Illumina^® Protocol (ChIP-seq, CUT&RUN) (Protocols)

DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN): easy to follow directions describing the step by step experimental procedure.

DNA Methylation (Pathway Diagram)

Expert-reviewed interactive diagram providing a current overview of DNA methylation, as well as links to products from CST.

Dopamine Signaling in Parkinson's Disease (Pathway Diagram)

Download Pathways by Disease Area (Pathway Diagram)

Resources include signaling pathways, antibodies and companion products, research overviews, technical resources, and recent review articles.

Dropping with the temps: Cool deals on CST mAbs

Education in Science Grants

CSTs commitment to Science Education: teacher grants for scientific education resources and summer internship programs.

EF-Hand Protein Domain (Protein Domains)

A scientific resource for the EF Hand protein domain containing information on structure, function, and calcium binding.

EH Protein Domain (Protein Domains)

A scientific resource for the EH protein domain containing information on structure, function, and domain binding during endocytosis and vesicle trafficking.

ELISA (Enzyme-linked Immunosorbent Assay) Detection Strategies

Three major categories of methods exist for detection in ELISA: colorimetric, fluorescent, and chemiluminescent. The type of substrate used depends on the needed assay sensitivity and on the signal-detection limits of the instrument used to read the plat

ELISA (Enzyme-linked Immunosorbent Assay) Test Results Interpretation

The following three types of analysis are used to analyze results from ELISA assays: qualitative, semiquantitative, and quantitative.

ELISA Protocol - Specific For Product: 12856 (Protocols)

ELISA Protocol for PathScan® Stress and Apoptosis Signaling Antibody Array Kit (Chemiluminescent Readout) #12856: easy to follow directions describing the step by step experimental procedure.

ELISA Protocol - Specific For Product: 12923 (Protocols)

ELISA Protocol for PathScan® Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout) #12923: easy to follow directions describing the step by step experimental procedure.

ELISA Resource Center

CST ELISA kits and reagents let you measure key cellular signaling nodes and other cellular target proteins using a wide selection of sandwich antibody assays.

ELISA-Peptide Assay Protocol (Protocols)

ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.

Employee Programs

Employee Volunteer Program

Engagement Committees and Events

ENTH Protein Domain (Protein Domains)

A scientific resource for the ENTH protein domain containing information on structure, function, and domain binding to phospholipids during endocytosis.

Environmental & Social Responsibility

The Corporate Social Responsibility of Cell Signaling Technology, Inc.

Environmental Grants

CST supports environmental projects and science education by providing small grants to local organizations.

Enzyme Immuno Assay Kit Protocol (Protocols)

Enzyme Immuno Assay Kit Protocol: easy to follow directions describing the step by step experimental procedure.

Enzyme-linked Immunosorbent Assay (ELISA) Components

In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.

Epigenetic Drivers and Regulators of Cancer

Epigenetic Drivers of Breast Cancer

Epigenetic Drivers of Leukemias

Epigenetic Drivers of Prostate Cancer

Epigenetic Writers and Erasers of Histone H3 (Pathway Diagram)

Epigenetic Writers and Erasers of Histones H2A, H2B, and H4 (Pathway Diagram)

Epigenetics (Pathway Diagram)

Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.

Equipment Reuse

ErbB/HER Signaling (Pathway Diagram)

Erk Antibody Comparison Table

Compare Cell Signaling Technology's Erk antibodies against one another to determine optimal applications

Erk Signaling (Product Type Overviews)

Phospho-Erk product lists, product citation lists, product selection tools, comparison tables and educational resources for MAPK signaling from Cell Signaling Technology.

EVH1 Protein Domain (Protein Domains)

A scientific resource for the EVH1 protein domain containing information on structure, function, and binding to a proline-rich motif to regulate actin dynamics.

Examples of Crosstalk Between Post-translational Modifications (Pathway Diagram)

Exceptional Performance of XP^® Monoclonal Antibodies (Product Type Overviews)

Case Study 4: Exceptional Performance of XP Monoclonal Antibodies in All Applications pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.

Exceptional Reproducibility of XP^® Monoclonal Antibodies (Product Type Overviews)

Case Study 3: Exceptional Reproducibility of XP® Monoclonal Antibodies in All Applications pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.

Exceptional Sensitivity of XP^® Monoclonal Antibodies (Product Type Overviews)

Case Study 2: Exceptional Sensitivity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.

Exceptional Specificity of XP^® Monoclonal Antibodies (Product Type Overviews)

Case Study 1: Exceptional Specificity of XP Monoclonal Antibodies pertaining to eXceptional Monoclonal Technology (XMT) from Cell Signaling Technology.

Experimental Design: Analyzing Intracellular Signaling With Flow Cytometry

Experimental Design: Controls for Immunofluorescence (IF)

F-Box Protein Domain (Protein Domains)

A scientific resource for the F-Box protein domain containing information on structure, function, and domain binding to ubiquitin in protein degradation pathways.

FastScan^® Sandwich ELISA Protocol (Colormetric ELISA) (Protocols)

FastScan™ ELISA (Enzyme-Linked Immunosorbent Assay) Kits

FastScan ELISA kits detect key signaling proteins using a solution-based assay format and are validated to ensure reproducible results across lots and users.

Feel the Flow: Immune Cell Signaling Cascades Reveal Phenotype and Function

FERM Protein Domain (Protein Domains)

A scientific resource for the FERM protein domain containing information on structure, function, and domain binding in cytoskeleton-associated proteins.

FF Protein Domain (Protein Domains)

A scientific resource for the FF protein domain containing information on structure, function, and binding to phospho-serine motifs.

FH2 Protein Domain (Protein Domains)

A scientific resource for the FH2 protein domain containing information on structure, function, and domain binding to actin.

FHA Protein Domain (Protein Domains)

A scientific resource for the FHA protein domain containing information on structure, function, and binding to phospho-serine and phospho-threonine motifs.

Fibrosis

Find alternatives to Santa Cruz antibodies

Use the lookup tool to find the CST equivalent for your SCBT product.

Flow Cytometry (F) Protocol for the Detection of Transcription Factors (Protocols)

Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.

Flow Cytometry BrdU Modified Protocol (Protocols)

Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Live Cell Protocol (Protocols)

Flow Cytometry Live Cell Protocol: easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Protocol (Flow) (Protocols)

Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Protocol for Extracellular Staining Using Conjugated Secondary Antibodies (Protocols)

Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC) (Protocols)

Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells (Protocols)

Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Resource Center (Application Overviews)

Flow cytometry resources and products from CST Cell Signaling Technology

Flow Cytometry Technical Support (Protocols)

Technical support questions and issues for flow cytometry-validated antibodies and products from CST Cell Signaling Technology

Flow Cytometry Troubleshooting Guide (Protocols)

Troubleshooting tips for successfully performing Intracellular Flow Cytometry using CST recommended protocols and antibodies validated for Flow.

Flow Cytometry, FoxP3/Transcription Factor Fixation and Permeabilization Protocol (Protocols)

Flow Cytometry, Methanol Permeabilization Protocol (Protocols)

Flow Cytometry, Methanol Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.

Flow Cytometry, Triton™ X-100 Permeabilization Protocol (Protocols)

Flow Cytometry, Triton™ X-100 Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.

Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification Protocol (Protocols)

Protocol for Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification

Fluorescent Western Blotting Resources

Western blotting using fluorescent-conjugated secondary antibodies - accurate quantification and two-color multiplexing using primary antibodies from different species.

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA) (Protocols)

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk) (Protocols)

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.

Formaldehyde vs. Alcohol Fixation for Immunofluorescence (IF)

Four Situations Where Flow Cytometry Can Help

Frequently Asked Questions

Frequently asked questions using our products while doing your research

FYVE Protein Domain (Protein Domains)

A scientific resource for the FYVE protein domain containing information on structure, function, and binding to phospholipids.

G-Protein-Coupled Receptors Signaling to MAPK/Erk (Pathway Diagram)

G1/S Checkpoint (Pathway Diagram)

G2M/DNA Damage Checkpoint (Pathway Diagram)

GAT Protein Domain (Protein Domains)

A scientific resource for the GAT protein domain containing information on structure, function, and domain binding to ubiquitin and GTP-bound ARF.

GEL Protein Domain (Protein Domains)

A scientific resource for the GEL protein domain containing information on structure, function, and domain binding to actin in cytoskeletal regulation.

General Inquiries

Generation and Validation of Anti-Linker Monoclonal Antibodies for the Surface Detection of scFv-based CARs

Genetics of Alzheimer’s disease (Pathway Diagram)

Get Technical Episode 1: Antibody Basics

Get your discount code for SARS-CoV-2 products

Global Giving and Disaster Relief

Global Perspectives on Hallmarks of Neurodegeneration

GLUE Protein Domain (Protein Domains)

A scientific resource for the GLUE protein domain containing information on structure, function, and binding to phospholipids in the regulation of vesicle trafficking.

Glutamine Metabolism (Pathway Diagram)

GPCR, Calcium, cAMP (Pathway Diagram)

Overview of GPCR, Calcium, cAMP Signaling Resources, interactive pathway diagrams, and other technical resources.

GRAM Protein Domain (Protein Domains)

A scientific resource for the GRAM protein domain containing information on structure, function, and phospholipid binding during vesicle trafficking.

Green Chemistry

Green Committee

Cell Signaling Technology supports environmental initiatives through an internal group who review ways for CST to be sustainable.

Greenhouse Gas and Energy Management

CST has an energy management plan to reduce our use of resources.

Greenhouse Gas Management

CST is committed to following the Paris Climate Agreement.

GRIP Protein Domain (Protein Domains)

A scientific resource for the GRIP protein domain containing information on structure, function, and domain binding to ARF family GTPases during vesicle trafficking.

GYF Protein Domain (Protein Domains)

A scientific resource for the GYF protein domain containing information on structure, function, and domain binding to proline-rich motifs.

Hallmarks of Antibody Validation: Binary Strategy

Hallmarks of Antibody Validation: Complementary Strategies

Hallmarks of Antibody Validation: Heterologous Strategy

Hallmarks of Antibody Validation: Multiple Antibody Strategy

Hallmarks of Antibody Validation: Orthogonal Strategy

Hallmarks of Antibody Validation: Ranged Strategy

Hallmarks of Neurodegeneration

HEAT Protein Domain (Protein Domains)

A scientific resource for the HEAT protein domain containing information on structure, function, and domain binding.

HECT Protein Domain (Protein Domains)

A scientific resource for the HECT protein domain containing information on structure, function, and domain binding in ubiquitin-mediated protein degradation.

Hedgehog Signaling (Pathway Diagram)

High Content Screening (Application Overviews)

Validated antibodies for high content screening from CST Cell Signaling Technology

Highly Sensitive Post-Translationally Modified Peptide Enrichment for LC-MS Analysis

Hippo Signaling (Pathway Diagram)

Histone Methylation (Pathway Diagram)

Histone Modification Table (Table Resources)

The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.

History of CST

How CUT&RUN Profiles Chromatin

How Does Antibody Diluent Affect Staining in Immunohistochemistry (IHC)?

How Does PTMScan^® Direct Proteomic Service Work? (Proteomics Overviews)

Overview of methodology and client deliverables for the PTMScan Direct Proteomic Service offered by Cell Signaling Technology.

How Much Antibody Should I use in ChIP assays?

How to Analyze Multiple Markers in Tissue Samples Using Multiplex IHC

Multiplex immunohistochemistry (mIHC): Perform in depth tissue analysis on a single slide to understand protein co-localization and spatial context

How to Choose Enzymatic or Sonication Protocol for ChIP

This webinar will focus on the importance of the immune regulatory context of the TME

How to Choose the Appropriate ChIP Protocol and Generate Reliable Data

How to Combine Surface & Intracellular Targets in Flow Cytometry

How to Fix and Permeabilize Cells for Flow Cytometry

How to Flow: Antibody Staining Walkthrough

How to Identify Senescence in Cells and Tissue

How to Incorporate Cell Pellet Controls in IHC Experiments

How to Optimize your Chromatin Fragmentation for ChIP

How To Perform Antigen Retrieval (AR) in Immunohistochemistry (IHC)

How to Select an Antibody for Immunoprecipitation

How to Sonicate Chromatin: Enzymatic ChIP Protocol

How to Sonicate Chromatin: Sonication ChIP Protocol

How to Split Adherent Cell Lines: Step-By-Step Demonstration

How to Split Suspension Cell Lines: Step-By-Step Demonstration

How We Select Our Antibody Targets (Process Explanations)

How CST responds to customer feedback as it relates to development of the product portfolio.

HRP Chemiluminescence ELISA-P (Protocols)

HRP Chemiluminescence ELISA-Pl: easy to follow directions describing the step by step experimental procedure.

Human Immune Cell Marker Guide (Pathway Diagram)

This interactive guide shows the key cell and functional state markers for tumor-infiltrating immune cells in humans.Web Location: Immunology and Inflammation

Human Oncogenes & Tumor Suppressor Genes (Table Resources)

Summary of human oncogenes and tumor suppressor genes sorted by signaling pathway.

Human Protein Kinases Overview (Kinome Diagram)

Interactive kinome diagram of the human protein kinases.

Human Tyrosine Protein Kinases (Kinome Diagram)

A kinome diagram showing the human tyrosine kinase group. This group of protein kinases comprises receptor and non-receptor tyrosine kinases.

Hypoxia Signaling (Pathway Diagram)

Identifying Myeloid Subsets Using Antibodies

IHC Protocols

IHC Tissue Controls & More

IHC 和 mIHC 在疾病研究诊断中的方法介绍及注意事项

Illustration of Motif Antibody Coverage

IMAC Service (Proteomics Overviews)

Quantitating Ser/Thr phosphorylation by Immobilized Metal Affinity Chromatography enrichment and analysis identifies thousands of sites with minimal sample.

Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Protocols)

Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experim

Immune Checkpoint Signaling in the Tumor Microenvironment (Pathway Diagram)

Immuno-Parallel Reaction Monitoring (iPRM) (Proteomics Overviews)

iPRM is a multiplexed LC/MS assay service that combines the specificity of mass spectrometry, the throughput of an immunoassay, and the power of multiplexing to accelerate the time-to-market of your drug and/or diagnostic test.

Immunofluorescence (IF) Resource Center (Application Overviews)

Immunofluorescence-validated antibodies from CST Cell Signaling Technology

Immunofluorescence (IF) Troubleshooting Guide

A helpful guide to help answer questions and troubleshoot common problems encountered in immunofluorescence.

Immunofluorescence Protocol for Biotin-Conjugated Antibodies (Protocols)

Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol for Cell-based Assays Requiring Methanol Fixation (Protocols)

Immunofluorescence Protocol wit Methanol Fixation (IF Methanol-fixed): easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol for Methanol Fixation (IF Methanol-fixed): easy to follow directions describin

Immunofluorescence Protocol for Labeling with BrdU Antibody (Protocols)

Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol with DOTMAC Permeabilization (Protocols)

Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experim

Immunofluorescence Protocol with Formaldehyde Fixation (Protocols)

Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol with Gelatin Blocking Step (Protocols)

Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimenta

Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer (Protocols)

Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by st

Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm) (Protocols)

Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step exp

Immunofluorescence Protocol with n-Octyl-Glucoside PermeabilizationTechnology (Protocols)

Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing th

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