Specific For: PathScan® Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout) #12923
Prepare 1X Detection Antibody Cocktail as follows:
For running only 1 slide: Dilute 150 µl of 10X Detection Antibody Cocktail with 1350 µl of Array Diluent Buffer.
For running 2 slides: Dilute 300 µl of 10X Detection Antibody Cocktail with 2700 µl of Array Diluent Buffer.
Prepare 1X DyLight 680®-linked Streptavidin as follows:
For running only 1 slide: Dilute 150 µl of 10X DyLight 680®-linked Streptavidin with 1350 µl of Array Diluent Buffer.
For running 2 slides: Dilute 300 µl 10X DyLight 680®-linked Streptavidin with 2700 µl of Array Diluent Buffer.
*Keep on ice and protect from light.
Insert the metal clip into the groove in the gasket and rotate the clip into the locked position. Ensure that the clip is on the same side as the orientation line on the slide.
NOTE: one of the clips has a small dot etched onto the upper rib to assist with pad designation (see slide assembly photos).
Add 100 µl Array Blocking Buffer to each well and cover with sealing tape. Incubate for 15 minutes at room temperature on an orbital shaker.
NOTE: Do not allow the pads to dry out until after step 14.
Wash 4 – 5 minutes with 100 µl 1X Array Wash Buffer as in step 8.
NOTE: From this point on, keep slide protected from light.
DyLight® is a registered trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
posted November 2013