IMPORTANT: Please refer to the APPLICATIONS section on the front page of product datasheet or product webpage to determine if this product is validated and approved for use in Flow Cytometry (F). Please see the product-specific Flow protocol on the product webpage for appropriate fixation and permeabilization conditions, and recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If live cell staining is desired, proceed to Immunostaining (Section D). Please refer to the product webpage and product-specific protocol to determine whether it is compatible with live cell staining.
NOTE: Please refer to the product-specific protocol on the product webpage for correct permeabilization conditions. Not all products are compatible with methanol permeabilization.
NOTE: Count cells using a hemocytometer or alternative method.
posted July 2009
revised July 2017