Specific for product: Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit #12581.
Component | dH2O Volume |
---|---|
G6PDH Substrate | 250 μL |
G6PDH Developer | 100 μL |
G6PDH positive control | 50 μL |
NADP | 100 μL |
G6PDH cofactor | 100 μL |
Number of Tests = 10 samples (n=3) + 3 positive controls
Note: DO NOT add G6PD substrate to the Negative Control Solution.
Total Detection Solution (μL) | Negative Control Solution (μL) | Positive Control Solution (μL) | |
---|---|---|---|
G6PD Substrate (40X) | 62.5 | 0 | 0 |
G6PD Developer (100X) | 25 | 3.3 | 0 |
G6PDH cofactor (100X) | 25 | 3.3 | 0 |
G6PD positive control (100X) | 0 | 0 | 1 |
NADP+ (100X) | 25 | 3.3 | 0 |
G6PD Assay Buffer (1X) | 2362.5 | 320 | 99 |
Total (μL) (With ~10% extra volume) | 2500 (30 Samples + 3 Positive Controls) | 330 | 100 |
Table 1: Example of Calculation for 10 Samples in a 96-well plate (All triplicates)
Total Detection Solution (μL) | Negative Control Solution (μL) | Positive Control Solution (μL) | |
---|---|---|---|
G6PD Substrate (40X) | 19 | 0 | 0 |
G6PD Developer (100X) | 7.5 | 1 | 0 |
G6PDH cofactor (100X) | 7.5 | 1 | 0 |
G6PD positive control (100X) | 0 | 0 | 1 |
NADP+ (100X) | 7.5 | 1 | |
G6PD Assay Buffer (1X) | 708.5 | 97 | 99 |
Total (μL) (With ~10% extra volume) | 750 (30 Samples + 3 Positive Controls) | 100 | 100 |
Table 2: Example Calculation for 10 samples in a 384-plate (All triplicates)
posted November 5, 2013