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8553
PMSF
Buffers & Dyes
Buffer

PMSF #8553

Citations (0)
Chemical Structure of PMSF

Product Usage Information

For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use. Typically, a final concentration of 1 mM provides sufficient protease protection.

Storage

Store lyophilized at Room Temperature, protected from direct light. In lyophilized form, the chemical is stable for 24 months. Once in solution, PMSF can be stored at -20° C for up to 3 months, protected from light.

Product Description

Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation. Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer (#9803) and RIPA Buffer (#9806). Molecular Formula: C7H7FO2S Molecular Weight: 174.19 CAS: 329-98-6
MW (kDa) 174.19
Molecular Formula C7H7FO2S

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 8553
Cat. # Size Qty. Price
8553S
34.84 mg