Cat. # | Size | Qty. | Price |
---|---|---|---|
60951S | 100 µl |
|
REACTIVITY | Vir |
SENSITIVITY | Endogenous |
MW (kDa) | 10 |
SOURCE | Rabbit |
Product Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#59329, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Virus
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp66 of SARS-CoV-2 ORF9b protein. Antibodies are purified by peptide affinity chromatography.
ORF9b (open reading frame 9b) is one of a variable number of so-called accessory proteins encoded by the genomes of coronaviruses such as SARS-CoV-2, the agent responsible for the COVID-19 global pandemic (1). Research studies have suggested that viral accessory proteins may not contribute directly to viral replication, but rather support viral infection by interfering with host defense mechanisms (2). For example, studies of SARS-CoV-1, the agent responsible for the 2003 SARS epidemic, revealed that SARS-CoV-1 ORF9b contributes to suppression of the host interferon (IFN) response, in part by interfering with key IFN effector proteins on the mitochondrial membrane (3). A conserved mechanism of action has since been described for ORF9b of SARS-CoV-2. Studies have shown that SARS-CoV-2 ORF9b localizes to the host mitochondrial membrane, and physically associates with TOM70, an adaptor protein within the MAVS/TRAF3/TRAF6/TOM70 protein complex on the mitochondrial membrane that promotes IFN signaling in response to viral infection (4,5).
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