The Histone Modification Table provides a referenced list of many known histone modifications, associated modifying enzymes, and proposed functions.
SimpleChIP® Kits and Antibodies validated in-house by our antibody development scientists correlated to positive and negative control primers.
Ensure equal sample loading with loading control antibodies from CST.
Histone modification antibodies are validated using a peptide array assay at CST
A list of recommended cell types and treatments to be used as positive and negative controls for activation state specific antibodies
Chromatin immunoprecipitation, or ChIP, is a technique that uses antibodies to isolate specific DNA-binding proteins along with the bound DNA fragments from cells and tissues.
Overview of epigenetic and chromatin regulation pathways, antibodies and related reagents, interactive pathway diagrams, and technical resources for epigenetics research.
Use our protocol compatibility table to understand which immune signaling and phenotyping antibodies will work together in your multicolor flow cytometry experiment.
Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.
PD-L1/PD1 immune checkpoint helps tumor cells evade detection by the immune system. Highly specific and sensitive PD-L1 antibody now available from CST.
Resources include signaling pathways, antibodies and companion products, research overviews, technical resources, and recent review articles.
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
Neurodegeneration refers to disorders of the CNS that lead to loss of neurons or glia, and is associated with age-related diseases, autoimmune attacks on the nervous system, or genetic mutations that affect the heath of CNS cells.
Sonication ChIP Kit Performance Comparison
CST provides multiple options for avoiding the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions that can damage both antibody epitopes and the integrity of the chromatin.
The ESC and Lineage Markers diagram provides an overview of ESC differentiation along lineage-specific pathways and links to products from CST.
CUT&RUN Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
Learn how we have validated and optimized our CUT&RUN Kit to yield better signal-to-noise ratios, require fewer cells to analyze protein-DNA interactions, and be compatible with tissue samples. Get up to speed on all the protocol improvements we have mad
SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a
