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HRP Chemiluminescence ELISA-P (Protocols)

HRP Chemiluminescence ELISA-Pl: easy to follow directions describing the step by step experimental procedure.

PathScan® ELISA (Enzyme-Linked Immunosorbent Assay) Kits (Product Type Overviews)

PathScan ELISA kits detect endogenous levels of key signaling proteins using a traditional sandwich-based assay format in phospho-specific or total protein options.

Immunohistochemistry Protocol - Paraffin for SignalStain® Boost Detection Reagent (Protocols)

Immunohistochemistry Protocol – Paraffin for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol - Paraffin for Boost Detection Reagent: easy to follow directions describing

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads) (Protocols)

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol - Frozen for SignalStain® Boost Detection Reagent (Protocols)

Immunohistochemistry Protocol - Frozen for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.

SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads) (Protocols)

SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure

Western Blotting Protocol (Protocols)

Western Immunoblotting Protocol: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol with DOTMAC Permeabilization (Protocols)

Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experim

Immunofluorescence Protocol for Cell-based Assays Requiring Methanol Fixation (Protocols)

Immunofluorescence Protocol wit Methanol Fixation (IF Methanol-fixed): easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol for Methanol Fixation (IF Methanol-fixed): easy to follow directions describin

Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm) (Protocols)

Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step exp

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads) (Protocols)

SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a

ELISA-Peptide Assay Protocol (Protocols)

ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol – Specific for Product: 2187 (Protocols)

Immunofluorescence Protocol – Specific for Product: 2187: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol with Gelatin Blocking Step (Protocols)

Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimenta

In-Cell Western Protocol (Protocols)

In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol without Permeabilization (Protocols)

Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol for Labeling with BrdU Antibody (Protocols)

Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (Fluorescent) (Protocols)

Western Blotting (Fluorescent) Protocol: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol for Biotin-Conjugated Antibodies (Protocols)

Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol with n-Octyl-Glucoside PermeabilizationTechnology (Protocols)

Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing th

Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer (Protocols)

Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by st

Immunofluorescence Protocol with Formaldehyde Fixation (Protocols)

Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.

Immunofluorescence Protocol with Saponin Permeabilization (Protocols)

Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Solutions for Consistent IHC Results: Primary and Secondary Antibodies, Diluent, Blocking Buffers, Chromogens, Controls, and More

Get the reagents and supplies for immunohistochemistry (IHC) protocols including antibodies, diluent, blocking buffer, chromogens, and controls

Immunohistochemistry Protocol (Frozen) (Protocols)

Immunohistochemistry Protocol (Frozen): easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (for Conformation and Light-Chain Specific and Anti-Biotin Secondary Antibodies) (Protocols)

Western Blotting-Conformation, LC Specific, Anti-Biotin Secondary Ab: easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (Primary Ab Incubation In BSA) (Protocols)

Western Blotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk) (Protocols)

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.

Western Blotting Protocol (Primary Ab Incubation In Milk) (Protocols)

Western Blotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA) (Protocols)

Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol - Specific For Product: 12692 (Protocols)

Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.

Flow Cytometry (F) Protocol for the Detection of Transcription Factors (Protocols)

Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.

Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells (Protocols)

Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Immunoblotting) (Protocols)

Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.

Nuclear Permeabilization Staining (Protocols)

Nuclear Permeabilization Staining: easy to follow directions describing the step by step experimental procedure.

PathScan® Sandwich ELISA Antibody Pair Protocol (Protocols)

PathScan® Sandwich ELISA Antibody Pair Protocol: easy to follow directions describing the step by step experimental procedure.

Principle of ELISA (Enzyme-linked Immunosorbent Assay) Test

The ELISA method has many variations, all of which rely on complex of an antigen and an antibody/enzyme conjugate. Signal is generated by turnover of substrate.

PathScan® Sandwich ELISA Protocol (Chemiluminescent) (Protocols)

PathScan® Sandwich ELISA Protocol (Chemiluminescent): easy to follow directions describing the step by step experimental procedure.

Flow Cytometry BrdU Modified Protocol (Protocols)

Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.

Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Protocols)

Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experim

Flow Cytometry, Methanol Permeabilization Protocol (Protocols)

Flow Cytometry, Methanol Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.

SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads) (Protocols)

SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Live Cell Protocol (Protocols)

Flow Cytometry Live Cell Protocol: easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol (For Analysis By Kinase Assay) (Protocols)

Immunoprecipitation Protocol (For Analysis By Kinase Assay): easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC) (Protocols)

Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) (Protocols)

Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.

Flow Cytometry, Triton™ X-100 Permeabilization Protocol (Protocols)

Flow Cytometry, Triton™ X-100 Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.

PathScan® Sandwich ELISA Protocol (Colormetric ELISA) (Protocols)

PathScan® Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.

SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads) (Protocols)

SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.

Enzyme-linked Immunosorbent Assay (ELISA) Components

In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.

Optimizing the ELISA (Enzyme-Linked Immunosorbent Assay) Test

Optimization is critical to obtaining reliable ELISA results. The design and development of an ELISA assay depends on the various components, including capture antibody, blocking buffer, diluent, sample concentration, and detection antibody

Flow Cytometry Protocol for Extracellular Staining Using Conjugated Secondary Antibodies (Protocols)

Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.

Protocol for Combined Staining of Intracellular Proteins and Cell Surface Markers in Blood (Protocols)

Protocol for Combined Staining of Intracellular Proteins and Cell Surface Markers in Blood: easy to follow directions describing the step by step experimental procedure.

Immunoprecipitation Protocol (For Native Protein) (Protocols)

Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.

Flow Cytometry Protocol (Flow) (Protocols)

Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry (IHC) Troubleshooting Guide & the Importance of Using Controls (Protocols)

Learn how to best interpret IHC staining and understand experiment results accurately.

Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060 (Protocols)

Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060: easy to follow directions describing the step by step experimental procedure.

KinomeView® Protocol (Protocols)

KinomeView® Protocol: easy to follow directions describing the step by step experimental procedure.

Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification Protocol (Protocols)

Protocol for Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification

Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3633 (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3625 (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific For Product: 3287 (Protocols)

Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197 (Protocols)

Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197: easy to follow directions describing the step by step experimental procedure.

Immunohistochemistry Protocol (Paraffin) - Specific for Product: 9319 (Protocols)

Immunohistochemistry Protocol (Paraffin) - Specific for Product: easy to follow directions describing the step by step experimental procedure.

Immunohistochemical Analysis

Learn how to best interpret IHC staining and understand experiment results accurately.

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