HRP Chemiluminescence ELISA-Pl: easy to follow directions describing the step by step experimental procedure.
PathScan ELISA kits detect endogenous levels of key signaling proteins using a traditional sandwich-based assay format in phospho-specific or total protein options.
Immunohistochemistry Protocol – Paraffin for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol - Paraffin for Boost Detection Reagent: easy to follow directions describing
SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol - Frozen for Boost Detection Reagent: easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
Western Immunoblotting Protocol: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with DOTMAC Permeabilization: easy to follow directions describing the step by step experim
Immunofluorescence Protocol wit Methanol Fixation (IF Methanol-fixed): easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol for Methanol Fixation (IF Methanol-fixed): easy to follow directions describin
Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Methanol Permeabilization: easy to follow directions describing the step by step exp
SimpleChIP® Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a
ELISA-Peptide Assay Protocol : easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol – Specific for Product: 2187: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Gelatin Blocking Step: easy to follow directions describing the step by step experimenta
In-Cell Western Protocol: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol without Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol for Labeling with BrdU Antibody: easy to follow directions describing the step by step experimental procedure.
Western Blotting (Fluorescent) Protocol: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol for Biotin-Conjugated Antibodies: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with n-Octyl-Glucoside Permeabilization: easy to follow directions describing th
Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by step experimental procedure.Immunofluorescence Protocol with Image-iT™ FX Signal Enhancer: easy to follow directions describing the step by st
Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.Immunofluorescence General Protocol: easy to follow directions describing the step by step experimental procedure.
Immunofluorescence Protocol with Saponin Permeabilization: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Get the reagents and supplies for immunohistochemistry (IHC) protocols including antibodies, diluent, blocking buffer, chromogens, and controls
Immunohistochemistry Protocol (Frozen): easy to follow directions describing the step by step experimental procedure.
Western Blotting-Conformation, LC Specific, Anti-Biotin Secondary Ab: easy to follow directions describing the step by step experimental procedure.
Western Blotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.
Western Blotting Protocol (Primary Ab Incubation In Milk): easy to follow directions describing the step by step experimental procedure.
Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol: easy to follow directions describing the step by step experimental procedure for product #12692.
Flow Cytometry (F) Protocol for the Detection of Transcription Factors with recommended companion products.
Flow Cytometry Protocol for FoxP3 on Murine Splenocyte T Cells: easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol Utilizing Magnetic Separation (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.
Nuclear Permeabilization Staining: easy to follow directions describing the step by step experimental procedure.
PathScan® Sandwich ELISA Antibody Pair Protocol: easy to follow directions describing the step by step experimental procedure.
The ELISA method has many variations, all of which rely on complex of an antigen and an antibody/enzyme conjugate. Signal is generated by turnover of substrate.
PathScan® Sandwich ELISA Protocol (Chemiluminescent): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry BrdU Modified Protocol: easy to follow directions describing the step by step experimental procedure.
Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experimental procedure.Immobilized IP Protocol / (For Analysis By Western Blot): easy to follow directions describing the step by step experim
Flow Cytometry, Methanol Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Live Cell Protocol: easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Analysis By Kinase Assay): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol for FoxP3 on Human Peripheral Blood Mononuclear Cells (PBMC): easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Analysis By Western Immunoblotting) : easy to follow directions describing the step by step experimental procedure.
Flow Cytometry, Triton™ X-100 Permeabilization Protocol: easy to follow directions describing the step by step experimental procedure.
PathScan® Sandwich ELISA Protocol Colormetric: easy to follow directions describing the step by step experimental procedure.
SimpleChIP® Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
In addition to the samples to be analyzed, other required materials in order to run a complete ELISA experiment include microplates, reagents, buffers, antibodies, and a plate reader.
Optimization is critical to obtaining reliable ELISA results. The design and development of an ELISA assay depends on the various components, including capture antibody, blocking buffer, diluent, sample concentration, and detection antibody
Flow Cytometry Protocol - Extracellular Staining - Conjugated Secondary Abs: easy to follow directions describing the step by step experimental procedure.
Protocol for Combined Staining of Intracellular Proteins and Cell Surface Markers in Blood: easy to follow directions describing the step by step experimental procedure.
Immunoprecipitation Protocol (For Native Protein): easy to follow directions describing the step by step experimental procedure.
Flow Cytometry Protocol: easy to follow directions describing the step by step experimental procedure.
Learn how to best interpret IHC staining and understand experiment results accurately.
Immunohistochemistry Protocol (Frozen) – Specific for Product: 4060: easy to follow directions describing the step by step experimental procedure.
KinomeView® Protocol: easy to follow directions describing the step by step experimental procedure.
Protocol for Fluorescent Multiplex Immunohistochemistry (mIHC) with Tyramide Signal Amplification
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin): easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific For Products: 2085, 3197: easy to follow directions describing the step by step experimental procedure.
Immunohistochemistry Protocol (Paraffin) - Specific for Product: easy to follow directions describing the step by step experimental procedure.
Learn how to best interpret IHC staining and understand experiment results accurately.