Mixed Lineage Leukemia (MLL)

Western blot analysis of extracts from MLL1 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using MLL1 (D6G8N) Rabbit mAb (Carboxy-terminal Antigen) (upper) and α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower). MLL1 WT and KO MEF were kindly provided by Dr. Ali Shilatifard of Northwestern University.

Western blot analysis of extracts from MLL1 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using MLL1 (D6G8N) Rabbit mAb (Carboxy-terminal Antigen) (upper) and α-Actinin (D6F6) XP^® Rabbit mAb #6487 (lower). MLL1 WT and KO MEF were kindly provided by Dr. Ali Shilatifard of Northwestern University.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells and either DOT1L (D1W4Z) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP Human PabPC1 Exon 1 Primers #77410, Human RPLP0 Exon 2 Primers, and SimpleChIP Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells and either DOT1L (D1W4Z) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP Human PabPC1 Exon 1 Primers #77410, Human RPLP0 Exon 2 Primers, and SimpleChIP Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and p300 (D2X6N) Rabbit mAb using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 5 ng enriched ChIP DNA using DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN) #56795, and sequenced on the Illumina NextSeq. The figure shows binding across FDPS gene.

Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and p300 (D2X6N) Rabbit mAb using SimpleChIP^® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 5 ng enriched ChIP DNA using DNA Library Prep Kit for Illumina (ChIP-seq, CUT&RUN) #56795, and sequenced on the Illumina NextSeq. The figure shows binding across FDPS gene.