Cat. # | Size | Qty. | Price |
---|---|---|---|
95830T | 1 Kit (4 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
ATRX (D1N2E) Rabbit mAb 14820 | 20 µl |
|
H | 280 | Rabbit IgG |
Daxx (25C12) Rabbit mAb 4533 | 20 µl |
|
H M R | 110 | Rabbit IgG |
Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb 13969 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Histone H3 (D1H2) XP® Rabbit mAb 4499 | 20 µl |
|
H M R Mk | 17 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Rab | Goat |
Product Information
ATRX (D1N2E) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1189 of human ATRX protein. Daxx (25C12) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Gln255 of Daxx protein. Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of histone H3 in which Lys9 is tri-methylated. Histone H3 (D1H2) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human histone H3 protein.
α-thalassemia/mental retardation X-linked (ATRX) is a transcriptional regulator and helicase that belongs to the SNF2 family of chromatin remodeling proteins (1,2). Together with its binding partner death-associated protein 6 (Daxx), ATRX acts as histone chaperone to deposit histone variant H3.3 at repetitive DNA sequences such as telomeric, pericentric, and ribosomal gene repeats (3-6). ATRX is involved in many nuclear functions that ensure proper sister chromatid cohesion during mitosis and chromosome alignment during meiosis (7,8). The ATRX transcriptional regulator also plays a role in the maintenance of telomere integrity and the regulation of gene expression during mammalian development by influencing DNA methylation patterns at high DNA repeat sequences (9,10). Mutations in the corresponding ATRX gene results in ATR-X syndrome, an X-linked disorder characterized by intellectual disabilities, craniofacial abnormalities, and mild α-thalassemia (11,12). Research studies indicate that the loss of ATRX protein occurs in numerous cancers, including pancreatic neuroendocrine tumors (PanNETs) and pediatric glioblastoma, where telomere maintenance occurs independently of telomerase (13-16).
Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (17). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (18,19). Nuclear interactions have since been observed with CENP-C (20), Pax3 (22), DNA methyltransferase I (21) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4, and Dek. Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (22). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets, including NF-κB and E2F-1 (23).
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